Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,260	G→A	260 (TAG→TAA)	yjjV →	putative DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,260	0	G	A	100.0%	131.4 / NA	42	260 (TAG→TAA)	yjjV	putative DNase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/22); total (20/22)

GGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAG  >  NC_000913/4614132‑4614393

gggCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAg                                                                                                                             <  1:951760/139‑1 (MQ=255)

         aaCCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCAcg                                                                                                                    <  1:1137834/139‑1 (MQ=255)

               ccGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCAt                                                                                                              <  1:427857/139‑1 (MQ=255)

                            cccGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgcg                                                                                                 >  1:1477480/1‑139 (MQ=255)

                                            gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCg                                                                                 >  1:659006/1‑139 (MQ=255)

                                             cTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGa                                                                                <  1:934097/139‑1 (MQ=255)

                                             cTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGa                                                                                >  1:356693/1‑139 (MQ=255)

                                               ttGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                                              <  2:356693/139‑1 (MQ=255)

                                               ttGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                                              <  2:1676483/139‑1 (MQ=255)

                                                       tGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc                                                                        >  2:453283/1‑137 (MQ=255)

                                                       tGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc                                                                        <  1:453283/137‑1 (MQ=255)

                                                               gAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATc                                                              <  1:1431740/139‑1 (MQ=255)

                                                                 aCCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAt                                                            <  2:30596/139‑1 (MQ=255)

                                                                          tGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAg                                                                                                                             >  1:1622638/1‑65 (MQ=255)

                                                                          tGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAg                                                                                                                             <  2:1622638/65‑1 (MQ=255)

                                                                                       gCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCtt                                                                           >  1:1353190/1‑102 (MQ=255)

                                                                                       gCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCtt                                                                           <  2:1353190/102‑1 (MQ=255)

                                                                                        cGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCg                                     <  2:659006/139‑1 (MQ=255)

                                                                                        cGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCg                                     <  1:1617093/139‑1 (MQ=255)

                                                                                            gCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGca                                                                                               <  1:1417158/77‑1 (MQ=255)

                                                                                            gCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGca                                                                                               >  2:1417158/1‑77 (MQ=255)

                                                                                            gCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAgg                                 >  2:609991/1‑139 (MQ=255)

                                                                                            gCTTAATAACACGTATACGCTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCATGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAgg                                 >  1:30923/1‑139 (MQ=255)

                                                                                                     cacGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTAt                                                               >  1:460543/1‑100 (MQ=255)

                                                                                                     cacGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTAt                                                               <  2:460543/100‑1 (MQ=255)

                                                                                                      acGTATACGCTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCATGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAgg                       <  2:30923/139‑1 (MQ=255)

                                                                                                       cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGc                      >  1:1416097/1‑139 (MQ=255)

                                                                                                       cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGc                      >  1:1310610/1‑139 (MQ=255)

                                                                                                                tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCAcg                                                                                                                    >  2:1301367/1‑36 (MQ=21)

                                                                                                                tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCAcg                                                                                                                    <  1:1301367/36‑1 (MQ=255)

                                                                                                                tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGcc                                           <  1:252085/109‑1 (MQ=255)

                                                                                                                tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGcc                                           >  2:252085/1‑109 (MQ=255)

                                                                                                                  tttAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgccgc           >  2:1574135/1‑139 (MQ=255)

                                                                                                                    tAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCAGAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt                                                  <  2:1193922/98‑1 (MQ=255)

                                                                                                                    tAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt                                                  >  1:1193922/1‑98 (MQ=255)

                                                                                                                       cGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg      >  2:1657462/1‑139 (MQ=255)

                                                                                                                         tGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                                                                                              >  2:761802/1‑49 (MQ=255)

                                                                                                                         tGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                                                                                              <  1:761802/49‑1 (MQ=255)

                                                                                                                          gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                                              >  2:157113/1‑64 (MQ=14)

                                                                                                                          gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                                              <  1:157113/64‑1 (MQ=14)

                                                                                                                          gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa   >  1:813191/1‑139 (MQ=255)

                                                                                                                           ccGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAg  <  1:1236395/139‑1 (MQ=255)

GGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAG  >  NC_000913/4614132‑4614393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A