Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
443,051 |
C→T |
*197* (TAG→TAA) |
yajL ← |
oxidative‑stress‑resistance chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 443,051 | 0 | C | T | 100.0%
| 40.2
/ NA
| 14 | *197* (TAG→TAA) | yajL | oxidative‑stress‑resistance chaperone |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/7); total (7/7) |
GAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACC > NC_000913/442917‑443167
|
gAGCGCGGTGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAAGGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact < 2:510294/139‑1 (MQ=255)
aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTc > 2:325011/1‑139 (MQ=255)
gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata < 1:325011/139‑1 (MQ=255)
aGCAGGGCTCTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGc > 1:562436/1‑139 (MQ=255)
cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACTAGGTGTGATGCCa < 2:562436/139‑1 (MQ=255)
gCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTc > 1:800942/1‑139 (MQ=255)
tataTCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact < 1:423994/72‑1 (MQ=255)
tataTCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact > 2:423994/1‑72 (MQ=255)
cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc < 1:78902/91‑1 (MQ=255)
cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc > 2:78902/1‑91 (MQ=255)
cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccg > 2:375691/1‑89 (MQ=255)
cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccg < 1:375691/89‑1 (MQ=255)
gCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc < 1:70237/139‑1 (MQ=255)
aCGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc < 1:179296/50‑1 (MQ=255)
aCGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc > 2:179296/1‑50 (MQ=255)
ccGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCAATTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc > 2:394940/1‑117 (MQ=255)
ccGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCAATTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc < 1:394940/117‑1 (MQ=255)
|
GAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACC > NC_000913/442917‑443167
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
