Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
443,051 |
C→T |
*197* (TAG→TAA) |
yajL ← |
oxidative‑stress‑resistance chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 443,051 | 0 | C | T | 100.0%
| 134.1
/ NA
| 42 | *197* (TAG→TAA) | yajL | oxidative‑stress‑resistance chaperone |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/20); total (22/20) |
AGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGG > NC_000913/442918‑443177
|
aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTc > 1:429983/1‑139 (MQ=255)
cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGATGTTTGCCGCATCCGACATTACTCGtaa < 2:987928/139‑1 (MQ=255)
tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCt > 1:137970/1‑139 (MQ=255)
gcgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCa > 1:39029/1‑139 (MQ=255)
cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTg < 1:94576/121‑1 (MQ=255)
cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTg > 2:94576/1‑121 (MQ=255)
tAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata > 1:1137167/1‑93 (MQ=255)
tAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata < 2:1137167/93‑1 (MQ=255)
cAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑Ct > 2:394337/1‑139 (MQ=255)
gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc < 1:1088879/105‑1 (MQ=255)
gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc > 2:1088879/1‑105 (MQ=255)
gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑Cttttt > 1:1042222/1‑139 (MQ=255)
aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccg > 2:997290/1‑100 (MQ=255)
aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccg < 1:997290/100‑1 (MQ=255)
ggattatCGCCGGTATTTTTTGGTTTTT‑CGTCCGCTCCTGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGcc < 2:216869/135‑1 (MQ=255)
atatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCcca < 2:137970/139‑1 (MQ=255)
tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata > 1:1001466/1‑77 (MQ=255)
tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata < 2:1001466/77‑1 (MQ=255)
tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCAt > 1:1010876/1‑96 (MQ=255)
tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCAt < 2:1010876/96‑1 (MQ=255)
gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCccaaccaa > 1:282650/1‑139 (MQ=255)
gCCCGTAATTTGTGGTTTTTACGTCGCATCT‑GTCAGAATGGGCGTTTGCCGCATTCCACCTTACTCGTAATAATTATAAATTCCTGCCGCCCTCACCCGTTGTGGTGCCCCCTCATGGGCCTTTTTACCGcccacccac > 1:216869/1‑138 (MQ=255)
cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact < 1:573652/65‑1 (MQ=255)
cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact > 2:573652/1‑65 (MQ=255)
gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGcc > 1:344834/1‑128 (MQ=255)
gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGcc < 2:344834/128‑1 (MQ=255)
gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCccaaccaa > 1:240933/1‑135 (MQ=255)
gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCccaaccaa < 2:240933/135‑1 (MQ=255)
ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTc > 2:37784/1‑139 (MQ=255)
gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCTCCATCACCAGTTGTGATGCCACTTCATGGG‑Cttttt > 1:459943/1‑104 (MQ=255)
gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCTCCATCACCAGTTGTGATGCCACTTCATGGG‑Cttttt < 2:459943/104‑1 (MQ=255)
gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa < 1:37784/139‑1 (MQ=255)
gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa < 2:429983/139‑1 (MQ=255)
tGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt < 2:282650/139‑1 (MQ=255)
gTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgcc < 1:288100/57‑1 (MQ=255)
gTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgcc > 2:288100/1‑57 (MQ=255)
gATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc > 1:520591/1‑56 (MQ=255)
gATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc < 2:520591/56‑1 (MQ=255)
gCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc < 1:394337/139‑1 (MQ=255)
gCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc > 2:517948/1‑139 (MQ=255)
aCGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCAc > 2:736098/1‑87 (MQ=255)
aCGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCAc < 1:736098/87‑1 (MQ=255)
aCGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg > 1:947401/1‑139 (MQ=255)
ccGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctgg < 2:1042222/139‑1 (MQ=255)
|
AGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGG > NC_000913/442918‑443177
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
