Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	738,853	G→A	254 (TAG→TAA)	ybfD →	H repeat‑associated putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	738,853	0	G	A	100.0%	112.2 / NA	36	254 (TAG→TAA)	ybfD	H repeat‑associated putative transposase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/17); total (19/17)

TCCGCTGAAAGAATTAAATAATCCCAAGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAGAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAA  >  NC_000913/738721‑738984

tCCGCTGAAAGAATTAAATAATCCCAAGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAggg                                                                                                                               <  2:732682/139‑1 (MQ=255)

                      cccAAGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCt                                                                                                         <  2:473282/139‑1 (MQ=255)

                          aGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTa                                                                                                                         <  2:612585/119‑1 (MQ=255)

                          aGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTa                                                                                                                         >  1:612585/1‑119 (MQ=255)

                               gACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGt                                                                                                >  2:624420/1‑139 (MQ=255)

                                aCAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTc                                                                                               >  1:305299/1‑139 (MQ=255)

                                         cAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCgggg                                                                                      >  1:145436/1‑139 (MQ=255)

                                                          cACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAg                                                                                                 >  2:706485/1‑111 (MQ=255)

                                                          cACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAg                                                                                                 <  1:706485/111‑1 (MQ=255)

                                                              gCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGt                                                                 <  2:305299/139‑1 (MQ=255)

                                                                   gaagaaACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGc                                                            <  1:142724/139‑1 (MQ=255)

                                                                              tCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGAt                                                 >  1:101459/1‑139 (MQ=255)

                                                                                  cATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGtt                                                                                                                    <  1:535963/68‑1 (MQ=255)

                                                                                  cATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGtt                                                                                                                    >  2:535963/1‑68 (MQ=255)

                                                                                         tttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTAt                                                                                                                        <  2:749886/57‑1 (MQ=255)

                                                                                         tttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTAt                                                                                                                        >  1:749886/1‑57 (MQ=255)

                                                                                          ttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACaaa                                     >  1:680515/1‑139 (MQ=255)

                                                                                          ttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACaaa                                     >  1:325237/1‑139 (MQ=255)

                                                                                           tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTacaa                                                       >  2:240119/1‑120 (MQ=255)

                                                                                           tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTacaa                                                       <  1:240119/120‑1 (MQ=255)

                                                                                            gCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTcc                                                               >  1:726550/1‑111 (MQ=255)

                                                                                            gCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTcc                                                               <  2:726550/111‑1 (MQ=255)

                                                                                                   cccTGATGAGCTTATTGATTTCACGTTTGAATAAAAAggg                                                                                                                               <  1:631749/40‑1 (MQ=17)

                                                                                                   cccTGATGAGCTTATTGATTTCACGTTTGAATAAAAAggg                                                                                                                               >  2:631749/1‑40 (MQ=17)

                                                                                                   cccTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                                         >  2:56280/1‑78 (MQ=255)

                                                                                                   cccTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                                         <  1:56280/78‑1 (MQ=255)

                                                                                                            gCTTATTGATTTCACGTTTGAATAAAAATGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTcc                                                               >  2:69936/1‑95 (MQ=255)

                                                                                                            gCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTcc                                                               <  1:69936/95‑1 (MQ=255)

                                                                                                            gCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCt                   <  2:325237/139‑1 (MQ=255)

                                                                                                            gCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCt                   >  1:243147/1‑139 (MQ=255)

                                                                                                             cTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTa                                                                                                                         <  2:596184/36‑1 (MQ=255)

                                                                                                             cTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTa                                                                                                                         >  1:596184/1‑36 (MQ=18)

                                                                                                                       tCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTcc        >  1:383751/1‑139 (MQ=255)

                                                                                                                       tCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTcc        >  1:201905/1‑139 (MQ=255)

                                                                                                                             ttGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTaaa  <  1:624420/139‑1 (MQ=255)

                                                                                                                             ttGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTaaa  <  2:243147/139‑1 (MQ=255)

TCCGCTGAAAGAATTAAATAATCCCAAGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAGAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAA  >  NC_000913/738721‑738984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A