Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	738,853	G→A	254 (TAG→TAA)	ybfD →	H repeat‑associated putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	738,853	0	G	A	100.0%	113.6 / NA	36	254 (TAG→TAA)	ybfD	H repeat‑associated putative transposase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/20); total (16/20)

TCCGCTGAAAGAATTAAATAATCCCAAGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAGAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCC  >  NC_000913/738721‑738978

tCCGCTGAAAGAATTAAATAATCCCAAGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAggg                                                                                                                         <  2:802989/139‑1 (MQ=255)

      gAAAGAATTAAATAATCCCAAGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGCCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTa                                                                                                                   <  2:685474/139‑1 (MQ=255)

                          aGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACg                                                                                               >  1:247531/1‑139 (MQ=255)

                          aGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACg                                                                                               >  2:160624/1‑139 (MQ=255)

                              tGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAg                                                                                           <  2:1003553/139‑1 (MQ=255)

                                      aCGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                                   <  2:462018/139‑1 (MQ=255)

                                      aCGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                                   <  2:247531/139‑1 (MQ=255)

                                      aCGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCg                                                                                   <  2:165863/139‑1 (MQ=255)

                                              aGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAg                                                                                                                           <  2:316009/91‑1 (MQ=255)

                                              aGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAg                                                                                                                           >  1:316009/1‑91 (MQ=255)

                                                         tCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTaaa                                                                >  1:888496/1‑139 (MQ=255)

                                                              gCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGt                                                           <  1:963446/139‑1 (MQ=255)

                                                               cAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTc                                                          <  1:364658/139‑1 (MQ=255)

                                                                  agaagaAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTg                                                       >  2:287005/1‑139 (MQ=255)

                                                                     agaaACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTACGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTa                                                    >  1:428616/1‑139 (MQ=255)

                                                                         aCCCGTCTTCATATTTTTTTCGGTGTCCATGATGAGCTTATTTATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTacaac                                                <  1:933922/139‑1 (MQ=38)

                                                                              tCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGAt                                           >  1:617573/1‑139 (MQ=255)

                                                                                     atTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTc                                    >  1:633374/1‑139 (MQ=255)

                                                                                         tttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGc                                                                                                                     <  1:797360/54‑1 (MQ=255)

                                                                                         tttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGc                                                                                                                     >  2:797360/1‑54 (MQ=255)

                                                                                         tttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGaaa                                                                                                         <  2:152415/66‑1 (MQ=255)

                                                                                         tttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGaaa                                                                                                         >  1:152415/1‑66 (MQ=255)

                                                                                          ttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAgg                                                                                                                          >  2:484032/1‑48 (MQ=255)

                                                                                          ttGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAgg                                                                                                                          <  1:484032/48‑1 (MQ=255)

                                                                                           tGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAat                              >  1:1012634/1‑139 (MQ=255)

                                                                                            gCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAata                             >  1:265502/1‑139 (MQ=255)

                                                                                              gATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATc                           <  2:888496/139‑1 (MQ=255)

                                                                                                tGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCAt                         <  2:1037519/139‑1 (MQ=255)

                                                                                                tGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCAt                         <  2:633374/139‑1 (MQ=255)

                                                                                                          gaGCTTATTGATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTACGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGt               <  2:428616/139‑1 (MQ=255)

                                                                                                                  tGATTTCATGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTtctc                    <  1:654453/126‑1 (MQ=255)

                                                                                                                  tGATTTCACGTTTGAATAAACAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTtctc                    >  2:654453/1‑126 (MQ=255)

                                                                                                                   gATTTCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTa      <  2:265502/139‑1 (MQ=255)

                                                                                                                       tCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTcc                                                         >  2:133298/1‑84 (MQ=255)

                                                                                                                       tCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTcc                                                         <  1:133298/84‑1 (MQ=255)

                                                                                                                       tCACGTTTGAATAAAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTcc  >  2:452775/1‑139 (MQ=255)

TCCGCTGAAAGAATTAAATAATCCCAAGCATGACAGTTACGCAATTAGTGAAAAGAGTCACGGCAGAGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAGCTTATTGATTTCACGTTTGAATAGAAAGGGCAGCTATGGTTAGAAACTACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCC  >  NC_000913/738721‑738978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A