Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	776,397	C→T	A19V (GCA→GTA)	tolA →	membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	776,397	0	C	T	100.0%	114.9 / NA	36	A19V (GCA→GTA)	tolA	membrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/19); total (17/19)

GCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAG  >  NC_000913/776284‑776527

gCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcg                                                                                                           >  2:818532/1‑139 (MQ=255)

   tttCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAATAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        <  1:201046/139‑1 (MQ=255)

   tttCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        <  1:95436/139‑1 (MQ=255)

   tttCCCTGGCTTAAAAGAGGGCGGGTAACAGGCGAACAGTTTTTGGAAACGGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        <  2:806912/139‑1 (MQ=255)

     tCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCt                                                                                                      <  1:179713/139‑1 (MQ=255)

                aaaGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCAt                                                                                                                          <  2:519663/108‑1 (MQ=255)

                aaaGAGAGCGGGTAACAGGCGAACAGTTATTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCAt                                                                                                                          >  1:519663/1‑108 (MQ=255)

                     gagCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        <  2:632183/121‑1 (MQ=255)

                     gagCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcggcg                                                                                                        >  1:632183/1‑121 (MQ=255)

                      agCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGttcgttcg                                                                                     >  1:425894/1‑139 (MQ=255)

                      agCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGttcgttcg                                                                                     >  1:465618/1‑139 (MQ=255)

                        cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                                   <  2:425894/139‑1 (MQ=255)

                        cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTCATCTGGAGTTCGTTCGAt                                                                                   <  2:465618/139‑1 (MQ=255)

                                     aaCAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcgg                                                                                                          <  2:800958/103‑1 (MQ=255)

                                     aaCAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTgcgg                                                                                                          >  1:800958/1‑103 (MQ=255)

                                                       agagTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAgttcgt                                                                                        <  2:510878/103‑1 (MQ=255)

                                                       agagTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAgttcgt                                                                                        >  1:510878/1‑103 (MQ=255)

                                                           tgtCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCggtgg                                                >  1:566442/1‑139 (MQ=255)

                                                                 aGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAat                                                                             >  2:231783/1‑104 (MQ=255)

                                                                 aGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAat                                                                             <  1:231783/104‑1 (MQ=255)

                                                                       ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGa                                    >  2:391724/1‑139 (MQ=255)

                                                                       ccGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGa                                    >  2:630086/1‑139 (MQ=255)

                                                                         gAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACg                                  <  1:818532/139‑1 (MQ=255)

                                                                            cAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTg                               >  2:296810/1‑139 (MQ=255)

                                                                               aaCGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCt                                                                                                      >  2:725318/1‑65 (MQ=255)

                                                                               aaCGACAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCt                                                                                                      <  1:725318/65‑1 (MQ=255)

                                                                                    cAAGCTCAAGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGtt                       >  2:54765/1‑139 (MQ=255)

                                                                                            aGCGGGCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAgg               >  2:364607/1‑139 (MQ=255)

                                                                                                 gCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCgg          <  1:54765/139‑1 (MQ=255)

                                                                                                 gCGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCgg          <  1:296810/139‑1 (MQ=255)

                                                                                                  cGATAATTATTTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGt         >  1:749726/1‑139 (MQ=255)

                                                                                                         tattTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAg  <  2:217948/139‑1 (MQ=255)

                                                                                                         tattTCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAg  <  2:749726/139‑1 (MQ=255)

                                                                                                         agtttCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAg  <  1:45320/137‑1 (MQ=255)

                                                                                                           tttCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                                   >  1:77269/1‑56 (MQ=255)

                                                                                                           tttCAGTAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                                                                                   <  2:77269/56‑1 (MQ=255)

GCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGAGGCGGCGGTGGTTCGTCCATCGACGCTGTCATGGTTGATTCAGGTGCGGTAGTTGAG  >  NC_000913/776284‑776527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A