Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
869,338 |
C→T |
P607S (CCG→TCG) |
gsiA → |
glutathione ABC transporter ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 869,338 | 0 | C | T | 100.0%
| 103.1
/ NA
| 33 | P607S (CCG→TCG) | gsiA | glutathione ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/15); total (18/15) |
TGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTG > NC_000913/869210‑869467
|
tGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTa < 1:369552/139‑1 (MQ=255)
gcAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCg < 1:193721/139‑1 (MQ=255)
gTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGca > 2:138076/1‑121 (MQ=255)
gTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGca < 1:138076/121‑1 (MQ=255)
ctgTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCg > 2:554265/1‑139 (MQ=255)
tgTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCgg > 2:638811/1‑139 (MQ=255)
aTCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACca > 1:265510/1‑89 (MQ=255)
aTCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACca < 2:265510/89‑1 (MQ=255)
tCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGc < 1:554265/139‑1 (MQ=255)
ccAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCaa < 1:70501/139‑1 (MQ=255)
gCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAagag < 2:150306/139‑1 (MQ=255)
aaTATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCt > 1:320054/1‑139 (MQ=255)
tatTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGt > 2:764805/1‑139 (MQ=255)
cTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGt > 2:337236/1‑139 (MQ=255)
cGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAgg > 1:564183/1‑104 (MQ=255)
cGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAgg < 2:564183/104‑1 (MQ=255)
tGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTaca < 2:64869/130‑1 (MQ=255)
tGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTaca > 1:64869/1‑130 (MQ=255)
tGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTgg > 2:99241/1‑139 (MQ=255)
cGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAAc > 2:354468/1‑59 (MQ=255)
cGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAAc < 1:354468/59‑1 (MQ=255)
gCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCaa > 1:715836/1‑107 (MQ=255)
gCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCaa < 2:715836/107‑1 (MQ=255)
aGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGAACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTggg > 2:687383/1‑139 (MQ=255)
tctcGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAgg > 1:666451/1‑82 (MQ=255)
tctcGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAgg < 2:666451/82‑1 (MQ=255)
cGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGa < 1:99241/139‑1 (MQ=255)
tGCAATGCGTCGGGTTGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAg > 2:518140/1‑139 (MQ=255)
aTGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTg > 1:743903/1‑96 (MQ=255)
aTGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTg < 2:743903/96‑1 (MQ=255)
tGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTg > 1:413222/1‑139 (MQ=255)
cGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAagag > 2:300094/1‑87 (MQ=255)
cGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAagag < 1:300094/87‑1 (MQ=255)
|
TGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTG > NC_000913/869210‑869467
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
