Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	869,338	C→T	P607S (CCG→TCG)	gsiA →	glutathione ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	869,338	0	C	T	100.0%	95.9 / NA	31	P607S (CCG→TCG)	gsiA	glutathione ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/19); total (12/19)

TAAATTACTGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGC  >  NC_000913/869202‑869472

tAAATTACTGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCg                                                                                                                                      >  2:362256/1‑139 (MQ=255)

     tACTGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCa                                                                                                                                 <  2:268294/139‑1 (MQ=255)

                           tGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCa                                                                                                           >  2:284558/1‑139 (MQ=255)

                                  tCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACg                                                                                                    <  2:309301/139‑1 (MQ=255)

                                          aCGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATg                                                                                            <  2:933664/139‑1 (MQ=255)

                                           cGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGc                                                                                           >  1:460153/1‑139 (MQ=255)

                                             aCCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGt                                                                                         <  2:132077/139‑1 (MQ=255)

                                                              tgTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCgg                                                                        >  2:486214/1‑139 (MQ=255)

                                                                tCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACAACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGgag                                                                      >  2:744783/1‑139 (MQ=255)

                                                                           tCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGc                                                           <  1:284558/139‑1 (MQ=255)

                                                                           tCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGc                                                           <  2:275696/139‑1 (MQ=255)

                                                                           tCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGc                                                           <  1:530848/139‑1 (MQ=255)

                                                                           tCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGc                                                           <  1:486214/139‑1 (MQ=255)

                                                                           tCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGc                                                           <  1:362256/139‑1 (MQ=255)

                                                                                gCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAagag                                                      <  2:830105/139‑1 (MQ=255)

                                                                                 cAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCg                                                                         <  2:341956/119‑1 (MQ=255)

                                                                                 cAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCg                                                                         >  1:341956/1‑119 (MQ=255)

                                                                                  aaTATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCt                                                    <  2:397404/139‑1 (MQ=255)

                                                                                         aTCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACAcc                                             <  2:460153/139‑1 (MQ=255)

                                                                                            tGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTa                                          <  1:744783/139‑1 (MQ=255)

                                                                                             gCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAg                                         <  1:169779/139‑1 (MQ=255)

                                                                                              cGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGt                                        >  2:332340/1‑139 (MQ=255)

                                                                                                tGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTgg                                      >  2:127609/1‑139 (MQ=255)

                                                                                                      agagGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTaca                                               >  1:379994/1‑124 (MQ=255)

                                                                                                      agagGTGGCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTaca                                               <  2:379994/124‑1 (MQ=255)

                                                                                                             gCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAAc                                                                                                                   >  2:983963/1‑49 (MQ=255)

                                                                                                             gCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAAc                                                                                                                   >  2:718081/1‑49 (MQ=255)

                                                                                                             gCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAAc                                                                                                                   <  1:718081/49‑1 (MQ=255)

                                                                                                             gCAGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAAc                                                                                                                   <  1:983963/49‑1 (MQ=255)

                                                                                                               aGCCGTCTCGTTGCAATGCGTCGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTggg                       <  1:332340/139‑1 (MQ=255)

                                                                                                                                  gtcgggtcggGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATg    <  2:523073/139‑1 (MQ=255)

                                                                                                                                   tcgggtcgggGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATgg   >  1:319257/1‑139 (MQ=255)

                                                                                                                                    cGGGTCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGc  >  2:52263/1‑139 (MQ=255)

TAAATTACTGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGC  >  NC_000913/869202‑869472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A