Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,024,703 |
A→G |
Q78R (CAG→CGG) |
helD → |
DNA helicase IV |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,024,703 | 0 | A | G | 100.0%
| 98.3
/ NA
| 28 | Q78R (CAG→CGG) | helD | DNA helicase IV |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/11); total (17/11) |
TTCAATCAATTACTGGCGATTCACTGTAAGCGCGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGCACTGGGGAAAATAAATGGCTGACGCGTG > NC_000913/1024588‑1024837
|
ttCAATCAATTACTGGCGATTGACTGTAAGCGCGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTACACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATg > 2:505064/1‑139 (MQ=255)
ttCAATCAATTACTGGCGATTCACTGTAAGCGCGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATg > 1:784127/1‑139 (MQ=255)
tcaatTACTGGCGATTCACTGTAAGCGCGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCAc > 1:841415/1‑139 (MQ=255)
tGGCGATTCACTGTAAGCGCGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGgcggcg < 2:841415/139‑1 (MQ=255)
gCGATTCACTGTAAGCGCGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGt > 2:314804/1‑139 (MQ=255)
aGCGCGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGAt > 1:378497/1‑139 (MQ=255)
cgcgGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGgcggcg > 1:251754/1‑122 (MQ=255)
cgcgGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGgcggcg < 2:251754/122‑1 (MQ=255)
ggTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCg < 2:658740/134‑1 (MQ=255)
ggTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCg > 1:658740/1‑134 (MQ=255)
gTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTggc < 2:807500/109‑1 (MQ=255)
gTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTggc > 1:807500/1‑109 (MQ=255)
tGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGa > 1:367713/1‑115 (MQ=255)
tGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGa < 2:367713/115‑1 (MQ=255)
ggggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTc < 2:784127/139‑1 (MQ=255)
ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGa > 1:465357/1‑92 (MQ=255)
ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGa < 2:465357/92‑1 (MQ=255)
ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTg > 1:40684/1‑139 (MQ=255)
ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTg > 1:782147/1‑139 (MQ=255)
aGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGc > 2:484728/1‑69 (MQ=255)
aGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGttt > 2:512997/1‑73 (MQ=255)
aGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCGGCGttt < 1:512997/73‑1 (MQ=255)
cACGGCACCGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGCACTgggg < 2:782147/139‑1 (MQ=255)
cGAATGGGGCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGCACTGGGGAaaataaa < 1:513208/139‑1 (MQ=255)
ggCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGgcggcg > 2:349680/1‑47 (MQ=255)
ggCGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGgcggcg < 1:349680/47‑1 (MQ=255)
cGAGACGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGCACTGGGGAAAATAAATGGCTGAcg > 1:421783/1‑139 (MQ=255)
aCGCGGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGCACTGGGGAAAATAAATGGCTGACGCGTg > 1:123236/1‑139 (MQ=255)
cgcgGCGTTTTTACCATCATCTTGATGCTCACTgg < 2:523117/35‑1 (MQ=255)
|
TTCAATCAATTACTGGCGATTCACTGTAAGCGCGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGCACTGGGGAAAATAAATGGCTGACGCGTG > NC_000913/1024588‑1024837
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
