Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,192	G→A	Q447Q (CAG→CAA)	ydgA →	DUF945 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,192	0	G	A	100.0%	88.0 / NA	28	Q447Q (CAG→CAA)	ydgA	DUF945 family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/15); total (13/15)

ACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGG  >  NC_000913/1691070‑1691273

aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                   >  1:198427/1‑139 (MQ=255)

aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                   >  2:356832/1‑139 (MQ=255)

aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                   <  1:827896/139‑1 (MQ=255)

aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                   <  1:606275/139‑1 (MQ=255)

                 ggCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGAGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATAcc                                                  >  1:442334/1‑139 (MQ=255)

                 ggCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGAGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATAcc                                                  <  2:442334/139‑1 (MQ=255)

                    aaCTGAGTTTATGACTCAGGTAGCGAAGGTGGAAGGTTATCCGGAAGATCACGCGACAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCTGTCTGACCACCTTGCAGGACAATACCATc                                               >  2:556411/1‑139 (MQ=255)

                         aGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                          <  2:198427/139‑1 (MQ=255)

                           tttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGTGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                <  2:515345/131‑1 (MQ=255)

                           tttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                >  1:515345/1‑131 (MQ=255)

                            ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAg                                       <  2:460284/139‑1 (MQ=255)

                                   tCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTg                                                              >  2:712501/1‑109 (MQ=255)

                                   tCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTg                                                              <  1:712501/109‑1 (MQ=255)

                                         aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCt                          <  1:575412/139‑1 (MQ=255)

                                            gAAGCTGGAAGGTTATTAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                          <  1:506271/120‑1 (MQ=255)

                                            gAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAGTGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                          >  2:506271/1‑120 (MQ=255)

                                               gCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGt                    <  1:356832/139‑1 (MQ=255)

                                                               gAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                <  2:406418/95‑1 (MQ=255)

                                                               gAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                >  1:406418/1‑95 (MQ=255)

                                                                 aGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATggg  >  2:28351/1‑139 (MQ=255)

                                                                       aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATg                                                                                <  1:575238/55‑1 (MQ=255)

                                                                       aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATg                                                                                >  2:575238/1‑55 (MQ=255)

                                                                                                  tGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                   >  2:32520/1‑41 (MQ=255)

                                                                                                  tGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                   <  1:32520/41‑1 (MQ=255)

                                                                                                     aGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTg                                   >  1:485186/1‑70 (MQ=255)

                                                                                                     aGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTg                                   <  2:485186/70‑1 (MQ=255)

                                                                                                           aTCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                          >  2:296940/1‑57 (MQ=255)

                                                                                                           aTCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                          <  1:296940/57‑1 (MQ=255)

ACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGG  >  NC_000913/1691070‑1691273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A