Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,313,739 |
(C)5→4 |
coding (252/2673 nt) |
rcsD → |
phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,313,735 | 0 | C | . | 100.0%
| 165.7
/ NA
| 38 | coding (248/2673 nt) | rcsD | phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (22/16); total (22/16) |
CGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATG > NC_000913/2313607‑2313859
|
cgcCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCg > 1:228716/1‑139 (MQ=255)
ccGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGc < 1:27679/139‑1 (MQ=255)
gCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAag > 1:586473/1‑139 (MQ=255)
aaaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGc < 2:842099/116‑1 (MQ=255)
aaaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGc < 2:599638/116‑1 (MQ=255)
aaaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGc > 1:599638/1‑116 (MQ=255)
aaaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGc > 1:842099/1‑116 (MQ=255)
aaaGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTg > 2:681469/1‑139 (MQ=255)
aTTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAg < 2:563919/108‑1 (MQ=255)
aTTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAg > 1:563919/1‑108 (MQ=255)
aTTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGt < 1:681469/139‑1 (MQ=255)
gCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAAcc > 2:501282/1‑139 (MQ=255)
cAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGc < 2:816301/80‑1 (MQ=255)
cAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGc > 1:816301/1‑80 (MQ=255)
aaaGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCa < 2:228716/139‑1 (MQ=255)
aaGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCaa > 2:755224/1‑139 (MQ=255)
gcgTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTC‑ACTATCTGGAGAAACCACGCCGCAAAACgg < 1:352516/139‑1 (MQ=255)
cgTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACgg > 2:689432/1‑139 (MQ=255)
cgTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACgg > 1:495203/1‑139 (MQ=255)
gTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGa < 1:436871/139‑1 (MQ=255)
cGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTcta > 1:620258/1‑97 (MQ=255)
cGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTcta < 2:620258/97‑1 (MQ=255)
aCCTGGCAGATCTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCa > 2:388941/1‑139 (MQ=255)
gATCTACGACAACATTGCCGCGACGACCT‑CCCGTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAAc < 1:560924/70‑1 (MQ=255)
gATCTACGACAACATCGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAAc > 2:560924/1‑70 (MQ=255)
cTACGACAACATTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCaa > 2:460706/1‑139 (MQ=255)
acaTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGAt < 1:687641/64‑1 (MQ=255)
acaTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGAt > 2:687641/1‑64 (MQ=255)
acaTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTcta > 2:342745/1‑69 (MQ=255)
acaTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTcta < 1:342745/69‑1 (MQ=255)
caTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGAcgc > 2:234989/1‑49 (MQ=255)
caTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGAcgc < 1:234989/49‑1 (MQ=255)
caTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCAcgccg < 2:70780/89‑1 (MQ=255)
caTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCAcgccg > 1:70780/1‑89 (MQ=255)
caTTGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTgaga > 2:170656/1‑139 (MQ=255)
ttGCCGCGACGACCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 2:79695/1‑139 (MQ=255)
acgacCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTAt < 1:437498/62‑1 (MQ=255)
acgacCT‑CCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTAt > 2:437498/1‑62 (MQ=255)
|
CGCCGTTAACGCCTGGCTGAAAGATAAAAGTTACCAGATTGTCGACATTACCCATGCTATCCAAAAGCGCGTCGATAACTGGCGTTACGTGACCTGGCAGATCTACGACAACATTGCCGCGACGACCTCCCCCTCCTCCGGCGAAGGTTTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATG > NC_000913/2313607‑2313859
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
