Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,405,627 |
(A)8→9 |
intergenic (‑555/+76) |
nuoA ← / ← lrhA |
NADH:ubiquinone oxidoreductase, membrane subunit A/transcriptional repressor of flagellar, motility and chemotaxis genes |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,405,619 | 1 | . | A | 100.0%
| 28.5
/ NA
| 11 | intergenic (‑547/+84) | nuoA/lrhA | NADH:ubiquinone oxidoreductase, membrane subunit A/transcriptional repressor of flagellar, motility and chemotaxis genes |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (6/5); total (6/5) |
TATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACT‑AAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGG > NC_000913/2405490‑2405747
|
tATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACT‑aaaaaaaaa > 1:485157/1‑138 (MQ=255)
tAACATCATTGTAGCAGTTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGTTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTg < 2:68229/139‑1 (MQ=255)
ggTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACAcccc > 2:165343/1‑139 (MQ=255)
ggTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACAcccc > 2:787236/1‑139 (MQ=255)
gTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGtctc > 1:262890/1‑139 (MQ=255)
cGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAgg > 1:495371/1‑139 (MQ=255)
gACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAAt < 2:262890/139‑1 (MQ=255)
aggTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAATACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTtcaatc < 2:495371/137‑1 (MQ=255)
gAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCtt < 2:782231/139‑1 (MQ=255)
ggATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTa < 1:120099/65‑1 (MQ=255)
ggATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTa > 2:120099/1‑65 (MQ=255)
gATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCGCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCgg > 2:337892/1‑139 (MQ=255)
|
TATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACT‑AAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGG > NC_000913/2405490‑2405747
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
