Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,405,627 |
(A)8→9 |
intergenic (‑555/+76) |
nuoA ← / ← lrhA |
NADH:ubiquinone oxidoreductase, membrane subunit A/transcriptional repressor of flagellar, motility and chemotaxis genes |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,405,619 | 1 | . | A | 100.0%
| 57.8
/ NA
| 19 | intergenic (‑547/+84) | nuoA/lrhA | NADH:ubiquinone oxidoreductase, membrane subunit A/transcriptional repressor of flagellar, motility and chemotaxis genes |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (10/9); total (10/9) |
TTGTAGCAGGTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACT‑AAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGC > NC_000913/2405505‑2405750
|
ttGTAGCAGGTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTaa > 1:911144/1‑139 (MQ=255)
gATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTg > 1:281808/1‑120 (MQ=255)
gATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTg < 2:281808/120‑1 (MQ=255)
attattTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTac < 2:267583/139‑1 (MQ=255)
gtggtTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAg > 1:321241/1‑121 (MQ=255)
gtggtTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAg < 2:321241/121‑1 (MQ=255)
ggtTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCg > 2:827765/1‑139 (MQ=255)
gtTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTg > 2:228345/1‑71 (MQ=255)
gtTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTg < 1:228345/71‑1 (MQ=255)
tGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTaaaa < 1:641224/79‑1 (MQ=255)
tGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTaaaa > 2:641224/1‑79 (MQ=255)
tCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGtct < 1:82890/89‑1 (MQ=255)
tCTGCACTATGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGtct > 2:82890/1‑89 (MQ=255)
tGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATcgt < 1:849224/97‑1 (MQ=255)
tGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATcgt > 2:849224/1‑97 (MQ=255)
tGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATcaaca < 1:430757/130‑1 (MQ=255)
tGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATcaaca > 2:430757/1‑130 (MQ=255)
tGCGATGAAGGATTTTTACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAatcat > 2:910778/1‑139 (MQ=255)
ttttACTAAAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGc < 2:545375/139‑1 (MQ=255)
|
TTGTAGCAGGTGATTTTTTTCAGGCGATTATTTGTGCGTTCGGGACGTGAATCTCTGGTGGTTGAAAAATGAACAGTTTTGTACGTTCTGCACTATGCGATGAAGGATTTTTACT‑AAAAAAAAGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGC > NC_000913/2405505‑2405750
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
