Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,137,229 |
A→G |
S143S (AGT→AGC) |
yijF ← |
DUF1287 family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,137,229 | 0 | A | G | 100.0%
| 100.4
/ NA
| 30 | S143S (AGT→AGC) | yijF | DUF1287 family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/16); total (14/16) |
TTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTT > NC_000913/4137097‑4137333
|
ttcCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTgggg < 2:516669/139‑1 (MQ=255)
cgcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTggg > 2:805781/1‑132 (MQ=255)
cgcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTg > 2:123553/1‑139 (MQ=255)
cgcgcCTGCGCCAATGTTATGTAGCCCCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTggg < 1:805781/132‑1 (MQ=255)
gcCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg < 1:24071/139‑1 (MQ=255)
ccAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc < 1:123553/139‑1 (MQ=255)
ccAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc < 1:81876/139‑1 (MQ=255)
aTGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCgtg < 2:258507/139‑1 (MQ=255)
cGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGAtt > 2:486402/1‑139 (MQ=255)
gAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGAc < 1:138781/139‑1 (MQ=255)
ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCTCCAGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATc > 2:2191/1‑139 (MQ=255)
ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACg < 2:610936/133‑1 (MQ=255)
ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACg > 1:610936/1‑133 (MQ=255)
gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGa > 1:123270/1‑108 (MQ=255)
gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGACGCTGGGGTTCTTGCTGGTggg < 2:522939/71‑1 (MQ=255)
gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGACGCTGGGGTTCTTGCTGGTggg > 1:522939/1‑71 (MQ=255)
gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGATTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGa < 2:123270/108‑1 (MQ=255)
cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCt < 1:733413/56‑1 (MQ=255)
cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCt > 2:733413/1‑56 (MQ=255)
cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTg > 1:531284/1‑58 (MQ=255)
cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTg < 2:531284/58‑1 (MQ=255)
cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCggg > 2:232883/1‑139 (MQ=255)
cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCggg < 1:232883/139‑1 (MQ=255)
gTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACtt > 1:591244/1‑139 (MQ=255)
cccGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg > 2:482441/1‑36 (MQ=255)
cccGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg < 1:482441/36‑1 (MQ=255)
ttGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTcc < 1:99997/64‑1 (MQ=255)
ttGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTcc > 2:99997/1‑64 (MQ=255)
aTAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGAt < 2:439219/83‑1 (MQ=255)
aTAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGAt > 1:439219/1‑83 (MQ=255)
|
TTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTT > NC_000913/4137097‑4137333
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
