Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,441,175 |
A→G |
V14A (GTA→GCA) |
ytfL ← |
UPF0053 family inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,441,175 | 0 | A | G | 100.0%
| 110.8
/ NA
| 32 | V14A (GTA→GCA) | ytfL | UPF0053 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/17); total (15/17) |
AAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAG > NC_000913/4441043‑4441299
|
aaGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGa < 2:600896/139‑1 (MQ=255)
gAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATc < 2:760015/139‑1 (MQ=255)
aTGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGc > 2:198396/1‑139 (MQ=255)
gATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt < 1:198396/139‑1 (MQ=255)
tCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATTGAGAAGAATGCACTTGCAGCGATCAAGCAGAGt < 2:387583/122‑1 (MQ=255)
tCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATTGAGAAGAATGCACTTGCAGCGATCAAGCAGAGt > 1:387583/1‑122 (MQ=255)
aCGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACa > 1:529866/1‑139 (MQ=255)
gcgTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCAGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACata < 2:472780/139‑1 (MQ=255)
gTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATc > 2:541470/1‑139 (MQ=255)
tataaatgcgCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg < 2:708978/91‑1 (MQ=255)
tataaatgcgCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg > 1:708978/10‑100 (MQ=255)
tttATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGa < 1:705630/139‑1 (MQ=255)
ttGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTc > 1:807714/1‑139 (MQ=255)
ccTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt > 1:550910/1‑98 (MQ=255)
ccTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt < 2:550910/98‑1 (MQ=255)
ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg > 1:494442/1‑88 (MQ=255)
ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg < 2:494442/88‑1 (MQ=255)
ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTg > 2:377865/1‑109 (MQ=255)
ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTg < 1:377865/109‑1 (MQ=255)
catcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATa < 2:114124/104‑1 (MQ=255)
catcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATa > 1:114124/1‑104 (MQ=255)
ccagcAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg > 1:387417/1‑80 (MQ=255)
ccagcAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg < 2:387417/80‑1 (MQ=255)
tttAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGAc < 2:439133/84‑1 (MQ=255)
tttAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGAc > 1:439133/1‑84 (MQ=255)
tGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGt > 2:428602/1‑71 (MQ=255)
tGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGt < 1:428602/71‑1 (MQ=255)
tGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg > 2:52573/1‑59 (MQ=255)
tGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg < 1:52573/59‑1 (MQ=255)
gAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTtgtgt < 1:278291/139‑1 (MQ=255)
cggcAAGCGAGATCGCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTCAAATACTGTTTAACATATCTTATCGGACCTTTCAGGCCAGATCCTCGGAAGCGGAAGTTGATTAATTTATGTGaaa < 1:512970/139‑1 (MQ=255)
agaagaACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCgaag > 1:26900/1‑139 (MQ=255)
|
AAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAG > NC_000913/4441043‑4441299
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
