Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,442,007 |
T→C |
E57G (GAG→GGG) |
msrA ← |
methionine sulfoxide reductase A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,442,007 | 0 | T | C | 100.0%
| 108.8
/ NA
| 31 | E57G (GAG→GGG) | msrA | methionine sulfoxide reductase A |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/18); total (13/18) |
CGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGG > NC_000913/4441873‑4442114
|
cGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCcac < 2:684596/139‑1 (MQ=255)
tGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca < 1:271955/129‑1 (MQ=255)
tGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCca > 2:271955/1‑129 (MQ=255)
tGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACAcccc < 2:582918/135‑1 (MQ=255)
tGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACAcccc > 1:582918/1‑135 (MQ=255)
gCGTACCGCTT‑GGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAaac > 1:730721/1‑139 (MQ=255)
ccGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAAcc > 1:629840/1‑139 (MQ=255)
tGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCaaaaa < 2:40799/139‑1 (MQ=255)
ggAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCAtt < 2:416391/139‑1 (MQ=255)
aGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTcc < 2:629840/139‑1 (MQ=255)
cccGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACAcccc > 1:194884/1‑93 (MQ=255)
cccGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACAcccc < 2:194884/93‑1 (MQ=255)
cccGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAg < 1:181222/135‑1 (MQ=255)
cccGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAg > 2:181222/1‑135 (MQ=255)
cGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACAt < 2:687562/139‑1 (MQ=255)
gTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGt < 2:684020/139‑1 (MQ=255)
cggcggTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGc < 2:721706/76‑1 (MQ=255)
cggcggTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGc > 1:721706/1‑76 (MQ=255)
gTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTAc < 2:7069/92‑1 (MQ=255)
gTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTAc > 1:7069/1‑92 (MQ=255)
gTAAACGCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCggg < 2:553851/139‑1 (MQ=255)
gCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGAc < 2:407781/105‑1 (MQ=255)
gCCGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGAc > 1:407781/1‑105 (MQ=255)
cGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGc < 1:451149/58‑1 (MQ=255)
cGGGTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGc > 2:451149/1‑58 (MQ=255)
gTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCa > 2:806044/1‑108 (MQ=255)
gTAACTGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCa < 1:806044/108‑1 (MQ=255)
tGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATg < 1:169180/101‑1 (MQ=255)
tGCCAGAACAGACGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATg > 2:169180/1‑101 (MQ=255)
aCGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAAtc > 1:240046/1‑43 (MQ=255)
aCGCCCCACACCCCAGAAACAACCCATCGCAAAAATGGCAAtc < 2:240046/43‑1 (MQ=255)
|
CGTAAACAATGCGTACCGCTTCGGCATGACCCGTATCACCGGAGCACACTTCCCGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGG > NC_000913/4441873‑4442114
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
