Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
494,419 |
G→A |
P5P (CCG→CCA) |
recR → |
gap repair protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 494,419 | 0 | G | A | 100.0%
| 88.2
/ NA
| 28 | P5P (CCG→CCA) | recR | gap repair protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/12); total (16/12) |
AAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCC > NC_000913/494284‑494549
|
aaGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTg > 1:1090000/1‑139 (MQ=255)
ccTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTaa > 2:1008234/1‑139 (MQ=255)
tggtggCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAaca < 1:838223/139‑1 (MQ=255)
tGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTa > 1:1003671/1‑139 (MQ=255)
ttCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGc < 2:1090000/139‑1 (MQ=255)
cgtATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCggg < 2:428833/139‑1 (MQ=255)
gaaACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGt < 2:951784/93‑1 (MQ=255)
gaaACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGt > 1:951784/1‑93 (MQ=255)
aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGaa > 2:895026/1‑139 (MQ=255)
tGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGt < 1:1011985/107‑1 (MQ=255)
tGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGt > 2:1011985/1‑107 (MQ=255)
cGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAAcaca > 1:398080/1‑65 (MQ=255)
cGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAAcaca < 2:398080/65‑1 (MQ=255)
cGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCg > 1:730718/1‑97 (MQ=255)
cGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCg < 2:730718/97‑1 (MQ=255)
ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt > 1:1039532/1‑108 (MQ=255)
ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt < 2:1039532/108‑1 (MQ=255)
aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGtt < 1:105657/125‑1 (MQ=255)
aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGtt > 2:105657/1‑125 (MQ=255)
cTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCg < 1:1008234/139‑1 (MQ=255)
gCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTgg > 1:918331/1‑139 (MQ=255)
ttCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCa > 2:10286/1‑139 (MQ=255)
aaCCGGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGcgcg < 1:963547/101‑1 (MQ=255)
aaCCGGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGcgcg > 2:963547/1‑101 (MQ=255)
aaCCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCAcc > 1:406381/1‑139 (MQ=255)
aCCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCAGTCTGCCGGGCGTTCGCCCGAAGTCGGCGCAGCGTATGGCGTTCTCGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCAccc > 2:747903/1‑139 (MQ=255)
gCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTc > 2:461435/1‑117 (MQ=255)
gCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTc < 1:461435/117‑1 (MQ=255)
|
AAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCC > NC_000913/494284‑494549
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
