Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	494,419	G→A	P5P (CCG→CCA)	recR →	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	494,419	0	G	A	100.0%	138.5 / NA	45	P5P (CCG→CCA)	recR	gap repair protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/20); total (25/20)

CTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGC  >  NC_000913/494289‑494553

cTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAac                                                                                                                                <  1:1061996/139‑1 (MQ=255)

                                 cgtATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCggg                                                                                               >  2:1111400/1‑139 (MQ=255)

                                      tGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAaca                                                                                                                               >  1:1671537/1‑102 (MQ=255)

                                      tGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAaca                                                                                                                               <  2:1671537/102‑1 (MQ=255)

                                      tGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTg                                                                                          >  1:1329335/1‑139 (MQ=255)

                                             aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGaa                                                                                   >  1:390984/1‑139 (MQ=255)

                                               gCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt                                                                                 <  1:1149780/139‑1 (MQ=255)

                                                   aaagaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgc                                                                             <  2:660967/139‑1 (MQ=255)

                                                      gaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCa                                                                          <  2:390984/139‑1 (MQ=255)

                                                             tGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATg                                                                   >  1:654311/1‑139 (MQ=255)

                                                                  tctGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACGGGCCCACTGTTAACTCAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGtt                                                              <  1:802942/139‑1 (MQ=255)

                                                                          ctccGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgctgc                                                      <  1:1111400/139‑1 (MQ=255)

                                                                           tccGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgctgct                                                     >  1:75164/1‑139 (MQ=255)

                                                                              ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgcg                                                                            <  1:1762461/113‑1 (MQ=255)

                                                                              ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgcg                                                                            >  2:1762461/1‑113 (MQ=255)

                                                                              ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgc                                                         <  2:1951936/132‑1 (MQ=255)

                                                                              ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgc                                                         >  1:1951936/1‑132 (MQ=255)

                                                                              ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                          <  2:949842/131‑1 (MQ=255)

                                                                              ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                          >  1:949842/1‑131 (MQ=255)

                                                                              ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCa                                                  >  2:180288/1‑139 (MQ=255)

                                                                                aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt                                                                                 <  1:876319/106‑1 (MQ=255)

                                                                                aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt                                                                                 >  2:876319/1‑106 (MQ=255)

                                                                                aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                          >  2:1980648/1‑129 (MQ=255)

                                                                                aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                          <  1:1980648/129‑1 (MQ=255)

                                                                                aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAgc                                                >  2:665219/1‑139 (MQ=255)

                                                                                aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAgc                                                >  2:1687669/1‑139 (MQ=255)

                                                                                  tGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCt                                                                                                                          <  1:808562/63‑1 (MQ=255)

                                                                                  tGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCt                                                                                                                          >  2:808562/1‑63 (MQ=255)

                                                                                  tGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAgcgc                                              >  2:330572/1‑139 (MQ=255)

                                                                                                 gCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCggg                               <  2:75164/139‑1 (MQ=255)

                                                                                                  cTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGa                              <  1:180288/139‑1 (MQ=255)

                                                                                                   tttAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGAt                             <  1:330572/139‑1 (MQ=255)

                                                                                                      aaGATGCCGTTCTGATGAAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCAGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCg                          >  2:1286515/1‑139 (MQ=255)

                                                                                                           gCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTgg                     >  1:775314/1‑139 (MQ=255)

                                                                                                           gCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTgg                     >  2:769617/1‑139 (MQ=255)

                                                                                                            ccGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGgc                    >  2:1009939/1‑139 (MQ=255)

                                                                                                              gTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGgcgc                  <  2:1329335/139‑1 (MQ=255)

                                                                                                               ttCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCa                 >  2:1979786/1‑139 (MQ=255)

                                                                                                                     tGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCt           <  1:1989299/139‑1 (MQ=255)

                                                                                                                       cAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACt                                                                                                              <  1:1165869/38‑1 (MQ=255)

                                                                                                                       cAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACt                                                                                                              >  2:1165869/1‑38 (MQ=255)

                                                                                                                         aaCCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgc                                                                             >  2:1939257/1‑69 (MQ=255)

                                                                                                                         aaCCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgc                                                                             <  1:1939257/69‑1 (MQ=255)

                                                                                                                            cAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCgg    >  2:703751/1‑139 (MQ=255)

                                                                                                                              gCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGc  >  2:925702/1‑139 (MQ=255)

CTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGC  >  NC_000913/494289‑494553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A