Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,371,482 |
(AT)4→3 |
coding (1267‑1268/1680 nt) |
ycjM → |
alpha amylase catalytic domain family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,371,476 | 0 | A | . | 100.0%
| 150.2
/ NA
| 36 | coding (1261/1680 nt) | ycjM | alpha amylase catalytic domain family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (20/16); total (20/16) |
| * | NC_000913 | 1,371,477 | 0 | T | . | 100.0%
| 150.2
/ NA
| 36 | coding (1262/1680 nt) | ycjM | alpha amylase catalytic domain family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (20/16); total (20/16) |
GTCCGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAG > NC_000913/1371351‑1371602
||
gTCCGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGat > 2:471252/1‑127 (MQ=255)
gTCCGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGat < 1:471252/127‑1 (MQ=255)
ccGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatattcaaag > 1:166568/1‑129 (MQ=255)
ccGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatattcaaag < 2:166568/135‑7 (MQ=255)
tATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGa < 1:121921/121‑1 (MQ=255)
tATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGa > 2:121921/1‑121 (MQ=255)
aaataaatGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCt < 2:492557/139‑1 (MQ=255)
atatGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatattcaaag > 2:253173/1‑107 (MQ=255)
atatGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatattcaaag < 1:253173/113‑7 (MQ=255)
tatGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGc > 1:1096434/1‑139 (MQ=255)
aTGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGgtg > 1:475668/1‑139 (MQ=255)
gCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCt > 2:485295/1‑116 (MQ=255)
gCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCt < 1:485295/116‑1 (MQ=255)
cGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCt > 1:958882/1‑115 (MQ=255)
cGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCt < 2:958882/115‑1 (MQ=255)
cGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCg > 1:1053294/1‑139 (MQ=255)
ttAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatattcaaag > 2:504944/1‑96 (MQ=255)
ttAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatattcaaag < 1:504944/102‑7 (MQ=255)
ttAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTgg < 2:24841/111‑1 (MQ=255)
ttAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTgg > 1:24841/1‑111 (MQ=255)
agTAGCGATGAAGGAAGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAAc > 2:756663/1‑139 (MQ=255)
aGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGt > 1:833248/1‑139 (MQ=255)
cGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatat < 1:497936/76‑1 (MQ=255)
cGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatat > 2:497936/1‑76 (MQ=255)
tGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatat < 1:102222/73‑1 (MQ=255)
tGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatat > 2:102222/1‑73 (MQ=255)
gaagaaCGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGAtt < 2:616696/139‑1 (MQ=255)
gaaCGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAAc > 1:365916/1‑139 (MQ=255)
cGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGa < 1:878936/108‑1 (MQ=255)
cGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGa > 2:937844/1‑108 (MQ=255)
cGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGa > 2:878936/1‑108 (MQ=255)
cGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGa < 1:937844/108‑1 (MQ=255)
tGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatat > 1:126142/1‑63 (MQ=255)
tGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatat < 2:126142/63‑1 (MQ=255)
tCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTa < 2:833248/139‑1 (MQ=255)
tGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatattcaa < 1:780170/50‑5 (MQ=255)
tGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGatatattcaa > 2:780170/1‑46 (MQ=255)
tGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATcaca > 2:289431/1‑132 (MQ=255)
tGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATcaca < 1:289431/132‑1 (MQ=255)
gCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAagag > 1:1005325/1‑139 (MQ=255)
ccATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAATAATATCACAGTAAAGAGAt < 1:756663/139‑1 (MQ=255)
cATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAAt > 1:521225/1‑67 (MQ=255)
cATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAAt < 2:521225/67‑1 (MQ=255)
aTGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATaa > 2:13930/1‑139 (MQ=255)
gCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAata < 1:791382/120‑1 (MQ=255)
gCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAata > 2:791382/1‑120 (MQ=255)
gCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAAcc > 1:116275/1‑139 (MQ=255)
tCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAAc > 1:1109693/1‑70 (MQ=255)
tCCCCGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAAc < 2:1109693/70‑1 (MQ=255)
ccGGTGTGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAg < 2:1096434/139‑1 (MQ=255)
gtgtGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGAt < 1:404300/132‑1 (MQ=255)
gtgtGCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGAt > 2:404300/1‑132 (MQ=255)
gtgCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATg < 2:439477/131‑1 (MQ=255)
gtgCCAGCG‑‑ATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATg > 1:439477/1‑131 (MQ=255)
||
GTCCGTATGAAATAAATGTGACCTATATGGATGCGTTAAGCCGCCGTGAGAGTAGCGATGAAGAACGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAG > NC_000913/1371351‑1371602
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
