Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,525,144 |
C→A |
L279F (TTG→TTT) |
ansP ← |
L‑asparagine transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,525,144 | 0 | C | A | 100.0%
| 198.4
/ NA
| 61 | L279F (TTG→TTT) | ansP | L‑asparagine transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (29/32); total (29/32) |
ACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGA > NC_000913/1525015‑1525278
|
aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata > 2:1068106/1‑139 (MQ=255)
aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata < 2:137232/139‑1 (MQ=255)
aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata < 2:222579/139‑1 (MQ=255)
aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACAGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata < 2:244141/139‑1 (MQ=255)
aaTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAg > 2:878206/1‑139 (MQ=255)
agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc < 2:440485/139‑1 (MQ=255)
agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc < 2:466136/139‑1 (MQ=255)
agGGCAGCCGTCAGCACCACAATGTTCATAATGCTGACGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc < 1:1068106/139‑1 (MQ=255)
ggCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCg > 2:935645/1‑139 (MQ=255)
cacaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCa < 1:313118/139‑1 (MQ=255)
tGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACg < 1:878206/139‑1 (MQ=255)
aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc > 2:971198/1‑112 (MQ=255)
aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc < 1:971198/112‑1 (MQ=255)
aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa > 2:503550/1‑139 (MQ=255)
aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa > 2:87623/1‑139 (MQ=255)
aaTGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATc > 2:579847/1‑139 (MQ=255)
aaTGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATc > 1:369779/1‑139 (MQ=255)
gctgcCGATATATGGCACACCCAGTTTAGAGAAGAACGTCACGAACGGACTTTGCCCCGCCTGATACGCACTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca > 2:160884/1‑139 (MQ=255)
gctgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca < 2:369779/139‑1 (MQ=255)
gctgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca < 1:1011547/139‑1 (MQ=255)
tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGc > 1:174846/1‑120 (MQ=255)
tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGc < 2:174846/120‑1 (MQ=255)
tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt > 1:2840/1‑139 (MQ=255)
tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt > 2:232743/1‑139 (MQ=255)
tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt > 1:119688/1‑139 (MQ=255)
tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACACCAAATCACACt > 2:344594/1‑139 (MQ=255)
atatGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg < 1:1051638/139‑1 (MQ=255)
atatGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACACCAAATCACACTGTTAATg < 1:344594/139‑1 (MQ=255)
ggCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAg > 1:41703/1‑80 (MQ=255)
ggCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAg < 2:41703/80‑1 (MQ=255)
acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc > 1:867623/1‑89 (MQ=255)
acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc < 2:867623/89‑1 (MQ=255)
acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc > 1:576677/1‑139 (MQ=255)
acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACATAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc > 1:613097/1‑139 (MQ=255)
aGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCa < 1:81900/139‑1 (MQ=255)
aGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCa < 2:25595/139‑1 (MQ=255)
tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa < 1:1128421/122‑1 (MQ=255)
tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa > 2:1128421/1‑122 (MQ=255)
agaAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCttt > 2:151531/1‑124 (MQ=255)
agaAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCttt < 1:151531/124‑1 (MQ=255)
agaAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTg > 1:696545/1‑139 (MQ=255)
aaaaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg < 1:935645/139‑1 (MQ=255)
aaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa < 2:2840/139‑1 (MQ=255)
aaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa < 1:579847/139‑1 (MQ=255)
aaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACATAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa < 2:613097/139‑1 (MQ=255)
cTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCgg < 2:119688/139‑1 (MQ=255)
tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAcc > 1:122484/1‑108 (MQ=255)
tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAcc < 2:122484/108‑1 (MQ=255)
gCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc < 2:868193/54‑1 (MQ=255)
gCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc > 1:868193/1‑54 (MQ=255)
gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa < 2:854036/88‑1 (MQ=255)
gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa > 1:854036/1‑88 (MQ=255)
gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTAc > 1:501102/1‑139 (MQ=255)
ccTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTAcc < 2:696545/139‑1 (MQ=255)
gATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACccac < 1:298455/139‑1 (MQ=255)
cACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGc < 2:1109726/119‑1 (MQ=255)
cACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGc > 1:1109726/1‑119 (MQ=255)
aCGGAAAAAACATAACCAGCAACACCACCGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGa > 2:534062/1‑139 (MQ=255)
cGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACg < 1:343662/54‑1 (MQ=255)
cGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACg > 2:343662/1‑54 (MQ=255)
cGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGcc > 1:708072/1‑56 (MQ=255)
|
ACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGA > NC_000913/1525015‑1525278
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
