Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,665,120 |
G→A |
*205* (TAG→TAA) |
dmsD → |
twin‑argninine leader‑binding protein for DmsA and TorA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,665,120 | 0 | G | A | 100.0%
| 149.5
/ NA
| 46 | *205* (TAG→TAA) | dmsD | twin‑argninine leader‑binding protein for DmsA and TorA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/21); total (25/21) |
ACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCG > NC_000913/1664985‑1665255
|
acGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGAt < 1:205168/139‑1 (MQ=255)
gATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAata > 2:293263/1‑139 (MQ=255)
aaCACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg > 2:479962/1‑139 (MQ=255)
aaCACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg < 1:479962/139‑1 (MQ=255)
ccGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaa < 2:343130/139‑1 (MQ=255)
gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTTCCg > 1:63335/1‑108 (MQ=255)
gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCg < 2:63335/108‑1 (MQ=255)
gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata > 1:536389/1‑139 (MQ=255)
gTGAACTGGCTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTCAATTCCTGTCGCGGTTAAACCGCTGTGTCGATAAGATAACAGTAATATTGCCGCATGCTGACGATTACGCGCAATCCAGGaata > 2:220387/1‑139 (MQ=255)
cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGAt < 2:615451/117‑1 (MQ=255)
cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGAt > 1:615451/1‑117 (MQ=255)
aCTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata > 1:1001341/1‑135 (MQ=255)
aCTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata < 2:1001341/135‑1 (MQ=255)
gggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCCATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGtt < 2:996477/139‑1 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCGATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt > 2:190720/1‑122 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTCcgcg > 1:732003/1‑118 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcgcg < 2:732003/118‑1 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt < 1:190720/122‑1 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCg > 1:948592/1‑125 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCg < 2:948592/125‑1 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaataat < 2:953673/133‑1 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa > 2:885531/1‑139 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa > 2:304075/1‑139 (MQ=255)
ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCAGGATGGTGACGATTACGCGCAATCCGGCaataat > 1:953673/1‑133 (MQ=255)
gAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAg > 2:340806/1‑139 (MQ=255)
aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt < 1:785448/117‑1 (MQ=255)
aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt > 2:785448/1‑117 (MQ=255)
aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCgg > 1:763441/1‑121 (MQ=255)
aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCgg < 2:763441/121‑1 (MQ=255)
tCGCTTAACGCTGGCGCAATGGCAGTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAata < 1:12046/83‑1 (MQ=255)
tCGCTTAACGCTGGCGCAATGGCAGTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAata > 2:12046/1‑83 (MQ=255)
cTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttt > 2:504797/1‑139 (MQ=255)
aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCa > 2:70701/1‑51 (MQ=255)
aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCa < 1:70701/51‑1 (MQ=255)
tCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCg < 2:576433/53‑1 (MQ=255)
tCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCg > 1:576433/1‑53 (MQ=255)
ttCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg < 1:340806/139‑1 (MQ=255)
tGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt > 2:600354/1‑103 (MQ=255)
tGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt < 1:600354/103‑1 (MQ=255)
gTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt > 2:71152/1‑68 (MQ=255)
gTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt < 1:71152/68‑1 (MQ=255)
gTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata > 2:539249/1‑71 (MQ=255)
gTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata < 1:539249/71‑1 (MQ=255)
gTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGTTTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAATCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGt > 1:189112/1‑139 (MQ=255)
tCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc < 2:565036/139‑1 (MQ=255)
aTAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCg > 2:186876/1‑139 (MQ=255)
|
ACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCG > NC_000913/1664985‑1665255
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
