Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,742,575 |
G→A |
intergenic (+14/+26) |
cfa → / ← ribC |
cyclopropane fatty acyl phospholipid synthase, SAM‑dependent/riboflavin synthase, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,742,575 | 0 | G | A | 100.0%
| 212.2
/ NA
| 65 | intergenic (+14/+26) | cfa/ribC | cyclopropane fatty acyl phospholipid synthase, SAM‑dependent/riboflavin synthase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/36); total (29/36) |
TTTAAACGAATGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGAT > NC_000913/1742439‑1742689
|
tttAAACGAATGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcc < 1:543573/139‑1 (MQ=255)
tAAACGAATGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcccc < 1:672771/139‑1 (MQ=255)
tttACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATAACCCCCTCTCCgggg < 2:731658/139‑1 (MQ=255)
attatCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGAttt > 2:820913/1‑139 (MQ=255)
tatCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCgggg < 1:174729/130‑1 (MQ=255)
tatCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCgggg > 2:174729/1‑130 (MQ=255)
tatCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCa > 1:1006754/1‑139 (MQ=255)
aaTGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAg < 2:841466/139‑1 (MQ=255)
tGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGc > 2:156006/1‑139 (MQ=255)
tGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGc > 1:317116/1‑139 (MQ=255)
ccTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt < 1:682323/139‑1 (MQ=255)
cTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTc > 2:907316/1‑139 (MQ=255)
gTGCTTTCCGCGCCCGTGATATTCAGCTCTTGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCt < 2:723559/132‑1 (MQ=255)
gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcccc < 2:836240/101‑1 (MQ=255)
gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAcccc > 1:836240/1‑101 (MQ=255)
gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCgggg < 1:464604/111‑1 (MQ=255)
gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCgggg > 2:464604/1‑111 (MQ=255)
gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCt > 1:723559/1‑132 (MQ=255)
gCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt < 2:1006754/139‑1 (MQ=255)
gCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt < 2:817413/139‑1 (MQ=255)
ttCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCgggg < 1:611182/106‑1 (MQ=255)
ttCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCgggg > 2:611182/1‑106 (MQ=255)
cgcgCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTc < 2:680387/139‑1 (MQ=255)
gcgcCCATGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCa < 2:1024348/139‑1 (MQ=255)
ctctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgcc > 2:528709/1‑139 (MQ=255)
ctctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgcc > 2:340860/1‑139 (MQ=255)
tctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGc > 1:715458/1‑109 (MQ=255)
tctGGCAGGTAGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGc < 2:715458/109‑1 (MQ=255)
gCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt < 1:308380/99‑1 (MQ=255)
gCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCtt > 2:308380/1‑99 (MQ=255)
cAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGc < 1:156006/139‑1 (MQ=255)
ggTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGcac < 2:188798/139‑1 (MQ=255)
gTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGcaca > 1:371260/1‑139 (MQ=255)
tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCa > 1:391363/1‑86 (MQ=255)
tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCa < 2:391363/86‑1 (MQ=255)
tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCt < 2:716731/93‑1 (MQ=255)
tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCt > 1:716731/1‑93 (MQ=255)
tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGtt < 2:371260/139‑1 (MQ=255)
ttCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCt > 2:695268/1‑139 (MQ=255)
tctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa < 1:340860/139‑1 (MQ=255)
tctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa < 2:842119/139‑1 (MQ=255)
tcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc < 1:907316/139‑1 (MQ=255)
aCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt < 2:885324/95‑1 (MQ=255)
aCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt > 1:885324/1‑95 (MQ=255)
cgGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTc > 1:199762/1‑98 (MQ=255)
cgGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTc < 2:199762/98‑1 (MQ=255)
tgtTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTAc > 2:260831/1‑139 (MQ=255)
aCGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCt < 1:528709/139‑1 (MQ=255)
cGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTg < 1:260831/139‑1 (MQ=255)
ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGc > 2:417031/1‑51 (MQ=255)
ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGc < 1:417031/51‑1 (MQ=255)
ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCTTGGTTGATTCATGGCATTTTCTCGt > 1:517724/1‑100 (MQ=255)
ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGt < 2:517724/100‑1 (MQ=255)
ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGa > 1:549508/1‑139 (MQ=255)
tGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGAt < 2:317116/139‑1 (MQ=255)
gCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCAt < 1:928185/75‑1 (MQ=255)
gCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCAt > 2:928185/1‑75 (MQ=255)
cGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGAt < 2:535073/134‑1 (MQ=255)
cGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGAt > 1:535073/1‑134 (MQ=255)
ggCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc > 2:892205/1‑99 (MQ=255)
ggCTATTCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc < 1:892205/99‑1 (MQ=255)
ttCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa < 1:341580/92‑1 (MQ=255)
ttCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa > 2:341580/1‑92 (MQ=255)
tCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt < 2:1033155/51‑1 (MQ=255)
tCTATCACCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt > 1:1033155/1‑51 (MQ=255)
|
TTTAAACGAATGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGAT > NC_000913/1742439‑1742689
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
