Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,923,535	T→C	N58D (AAT→GAT)	yebY ←	DUF2511 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,923,535	0	T	C	100.0%	118.8 / NA	34	N58D (AAT→GAT)	yebY	DUF2511 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/17); total (17/17)

CATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGAACTGGT  >  NC_000913/1923407‑1923670

cATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATAc                                                                                                                               <  1:959253/139‑1 (MQ=255)

       tCGCCGGGATTGTTAGGATCATCAATCCGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCAt                                                                                                                        >  1:679660/1‑139 (MQ=255)

        cGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCAtt                                                                                                                       >  2:620087/1‑139 (MQ=255)

                      ggATCATCAATCCGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAg                                                                                                         <  2:679660/139‑1 (MQ=255)

                           atcaATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACa                                                                                                                                  <  1:487322/109‑1 (MQ=255)

                           atcaATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACa                                                                                                                                  >  2:487322/1‑109 (MQ=255)

                                 cTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACa                                                                                                                              <  1:1002117/107‑1 (MQ=255)

                                 cTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACa                                                                                                                              >  2:1002117/1‑107 (MQ=255)

                                     aTCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCgg                                                                                                             >  1:467380/1‑120 (MQ=255)

                                     aTCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCgg                                                                                                             <  2:467380/120‑1 (MQ=255)

                                                   ttGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGcc                                                                            >  1:165699/1‑139 (MQ=255)

                                                       gCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCa                                                                          >  2:772514/1‑139 (MQ=255)

                                                       gCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAtt                                                                        <  2:693039/139‑1 (MQ=255)

                                                           tGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTc                                                                    >  1:1033266/1‑139 (MQ=255)

                                                            ggTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCt                                                                   <  2:1033266/139‑1 (MQ=255)

                                                                ttCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACa                                                                                                                              <  2:994859/76‑1 (MQ=255)

                                                                ttCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACa                                                                                                                              >  1:994859/1‑76 (MQ=255)

                                                                ttCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTAc                                                               >  1:1131157/1‑139 (MQ=255)

                                                                        ggCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCa                                                       >  1:791420/1‑139 (MQ=255)

                                                                        ggCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCa                                                       >  1:584987/1‑139 (MQ=255)

                                                                          cAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCaaa                                                     <  2:165699/139‑1 (MQ=255)

                                                                              ttCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGa                                                 <  2:1131157/139‑1 (MQ=255)

                                                                                         tgCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTa                                        <  1:200213/139‑1 (MQ=255)

                                                                                                   tttAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCg                              >  1:600654/1‑139 (MQ=255)

                                                                                                    ttAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCgg                             <  2:791420/139‑1 (MQ=255)

                                                                                                        aGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGca                         >  1:785626/1‑139 (MQ=255)

                                                                                                        aGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGca                         <  1:772514/139‑1 (MQ=255)

                                                                                                                  aCGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGcgccg               >  1:790335/1‑139 (MQ=255)

                                                                                                                    gAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCt             >  2:91151/1‑139 (MQ=255)

                                                                                                                       ggTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGt                                                                     <  2:856487/80‑1 (MQ=255)

                                                                                                                       ggTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGt                                                                     >  1:856487/1‑80 (MQ=255)

                                                                                                                       ggTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCa          <  2:584987/139‑1 (MQ=255)

                                                                                                                             cGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGAACTg    <  2:600654/139‑1 (MQ=255)

                                                                                                                               gATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGAACTGGt  <  2:785626/139‑1 (MQ=255)

CATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTT‑‑TGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGAACTGGT  >  NC_000913/1923407‑1923670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A