Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A1 F1 I1 R1 | 773 | 79.9 | 4112934 | 92.2% | 3792125 | 104.5 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,923,535 | T→C | N58D (AAT→GAT) | yebY ← | DUF2511 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,923,535 | 0 | T | C | 98.0% | 179.5 / ‑5.2 | 51 | N58D (AAT→GAT) | yebY | DUF2511 family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (24/26); minor base G (1/0); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGAAC > NC_000913/1923399‑1923666 | gCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaa > 1:478811/1‑139 (MQ=255)gCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaa > 2:1635981/1‑139 (MQ=255) cATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATAc < 1:1635981/139‑1 (MQ=255) tcaATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAg < 2:1736727/133‑1 (MQ=255) tcaATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAg > 1:1736727/1‑133 (MQ=255) caATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaata < 1:1705905/104‑1 (MQ=255) caATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaata > 2:1705905/1‑104 (MQ=255) caATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaat < 2:774437/103‑1 (MQ=255) caATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaat > 1:774437/1‑103 (MQ=255) tCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCAc > 1:545026/1‑139 (MQ=255) tGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACa < 1:1229052/106‑1 (MQ=255) tGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACa > 2:1229052/1‑106 (MQ=255) aaTCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACctct > 1:197864/1‑139 (MQ=255) cGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaataa > 2:2051868/1‑92 (MQ=255) cGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaataa < 1:2051868/92‑1 (MQ=255) aTGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCAt < 2:3204/122‑1 (MQ=255) aTGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCAt > 1:3204/1‑122 (MQ=255) ggTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACg < 2:545026/139‑1 (MQ=255) ggCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAt > 1:1922482/1‑139 (MQ=255) ggCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAt > 2:56957/1‑139 (MQ=255) ggCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAt < 2:1011372/139‑1 (MQ=255) ggCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAt < 1:56957/139‑1 (MQ=255) gCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAtt < 2:1339971/139‑1 (MQ=255) gCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAtt < 1:1061807/139‑1 (MQ=255) tGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTc > 2:1514140/1‑139 (MQ=255) tGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTc > 2:319990/1‑139 (MQ=255) ttCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCtt < 2:1629470/112‑1 (MQ=255) ttCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCtt > 1:1629470/1‑112 (MQ=255) tCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTAcc < 1:1514140/139‑1 (MQ=255) cccACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACg > 2:1771797/1‑122 (MQ=255) cccACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACg < 1:1771797/122‑1 (MQ=255) tGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaata > 2:375214/1‑62 (MQ=255) tGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaata < 1:375214/62‑1 (MQ=255) aCTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaat < 2:1363416/56‑1 (MQ=255) aCTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATcaat > 1:1363416/1‑56 (MQ=255) ttttGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGa < 2:1123688/139‑1 (MQ=255) tgCGATATGGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTa > 2:1338332/1‑139 (MQ=255) atatCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCAc < 2:417094/80‑1 (MQ=255) atatCGTTTAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCAc > 1:417094/1‑80 (MQ=255) tttAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGc > 1:1829750/1‑61 (MQ=255) tttAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGc < 2:1829750/61‑1 (MQ=255) tttAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTaa < 1:2014213/88‑1 (MQ=255) tttAAAGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTaa > 2:2014213/1‑88 (MQ=255) aaaGGATACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTg > 1:1446066/1‑139 (MQ=255) tACTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGcagcca > 2:1297065/1‑139 (MQ=255) aCTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAg < 1:1939359/54‑1 (MQ=255) aCTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAg > 2:1939359/1‑54 (MQ=255) aCTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGcagccag < 2:924621/139‑1 (MQ=255) aCTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGcagccag < 2:1922482/139‑1 (MQ=255) aCTGCACGAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGcagccag > 1:924621/1‑139 (MQ=255) cACGAGGGTACTCGGATGAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATCAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGcgcc > 1:1181311/1‑139 (MQ=255) gAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTAcc > 2:28556/1‑90 (MQ=255) gAGGGTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTAcc < 1:28556/90‑1 (MQ=255) gggTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCt < 1:503311/117‑1 (MQ=255) gggTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCt > 2:503311/1‑117 (MQ=255) ggTACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAACTCAGCATCACCTCTTCGCGATTAAACGCCCACTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCa < 1:1338332/139‑1 (MQ=255) tACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAt > 2:409903/1‑74 (MQ=255) tACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCAt < 1:409903/74‑1 (MQ=255) tACTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGa > 2:1472608/1‑139 (MQ=255) cTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTcgcg > 2:369669/1‑59 (MQ=255) cTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTcgcg < 1:369669/59‑1 (MQ=255) cTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGAAc < 2:1446066/139‑1 (MQ=255) cTCGGATCAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGAAc < 2:1181311/139‑1 (MQ=255) | GCCAGACTCATTTTTTCGCCGGGATTGTTAGGATCATCAATCTGAATCACCGAAATGGGTTGGGCATTGGTCTTCCCACTGGCAACTTCCTTTTGTGCGATATCGTTTAAAGGATACTGCACGAGGGTACTCGGATTAATAACATACAAAGCATTACCCGGTCGGCAAGTCAGCATCACCTCTTCGCGATTAAACGCCCATTTGTCTTTACCCACTTCAAAACGACTGACGGTAATCACCTGCGGTGCAGCCAGCGCCGCTGCAGAAC > NC_000913/1923399‑1923666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |