Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 765 46.1 2269152 93.8% 2128464 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,706,879 T→C V82V (GTA→GTG)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,706,8790TC100.0% 144.2 / NA 41V82V (GTA→GTG) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

TTCGAGATCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCACGCTCACGCTCAAGATCCATGGAATCGAGAACCTGCG  >  NC_000913/2706750‑2707012
                                                                                                                                 |                                                                                                                                     
ttCGAGATCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACACCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc                                                                                                                              >  1:831310/1‑139 (MQ=255)
    agaTCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCgggg                                                                                                                          <  2:479122/139‑1 (MQ=255)
         cATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCg                                                                                                                     >  2:431845/1‑139 (MQ=255)
            ttCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc                                                                                                                              <  2:807778/127‑1 (MQ=255)
            ttCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc                                                                                                                              >  1:807778/1‑127 (MQ=255)
                 tGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGAGTCGATAAAGtt                                                                                                             >  2:380982/1‑139 (MQ=255)
                       tgtAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTg                                                                                                       >  2:1049496/1‑139 (MQ=255)
                                 tGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCg                                                                                             >  1:805692/1‑139 (MQ=255)
                                 tGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCg                                                                                             >  1:339055/1‑139 (MQ=255)
                                   ccAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGcc                                                                                           >  1:548182/1‑139 (MQ=255)
                                        gTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAg                                                                                      <  1:431845/139‑1 (MQ=255)
                                                  cTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCAc                                                                                                                                     <  2:1121080/82‑1 (MQ=255)
                                                   tACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCtt                                                                                <  2:534983/134‑1 (MQ=255)
                                                   tACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCtt                                                                                >  1:534983/1‑134 (MQ=255)
                                                     cGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGt                                                                                         <  1:1111151/123‑1 (MQ=255)
                                                     cGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGt                                                                                         >  2:1111151/1‑123 (MQ=255)
                                                            ctcGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTc                                                                  >  2:690854/3‑139 (MQ=255)
                                                            cccGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCg                                                                                             <  1:278812/112‑1 (MQ=255)
                                                            cccGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCg                                                                                             >  2:278812/1‑112 (MQ=255)
                                                            cccGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTc                                                                  >  1:531611/1‑139 (MQ=255)
                                                                 cGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgct                                                             <  1:128083/139‑1 (MQ=255)
                                                                    cGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttg                                                          <  1:785503/139‑1 (MQ=255)
                                                                      accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCt                                                                                 <  1:260337/114‑1 (MQ=255)
                                                                      accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCt                                                                                 >  2:260337/1‑114 (MQ=255)
                                                                      accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttgcg                                                        >  1:865837/1‑139 (MQ=255)
                                                                      accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttgcg                                                        <  2:865837/139‑1 (MQ=255)
                                                                      accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttgcg                                                        <  2:491724/139‑1 (MQ=255)
                                                                      accaccAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttgcg                                                        >  1:491724/1‑139 (MQ=255)
                                                                           caGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCTCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttg                                                          <  2:586152/132‑1 (MQ=255)
                                                                           caGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCTCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCAcgctttg                                                          >  1:586152/1‑132 (MQ=255)
                                                                                        tCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCcac                                      >  1:139333/1‑139 (MQ=255)
                                                                                           caGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc                                                                                                                              >  2:930527/1‑48 (MQ=255)
                                                                                           caGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGccc                                                                                                                              <  1:930527/48‑1 (MQ=255)
                                                                                           caGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCcacgct                                   <  1:1049496/139‑1 (MQ=255)
                                                                                                  ccagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGa                                                                                                                    <  2:24144/51‑1 (MQ=255)
                                                                                                  ccagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGa                                                                                                                    >  1:24144/1‑51 (MQ=255)
                                                                                                  ccagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCacgctcacgctc                            <  2:831310/139‑1 (MQ=255)
                                                                                                   cagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCAcgctcacgctca                           <  2:339055/139‑1 (MQ=255)
                                                                                                   cagcGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCAcgctcacgctca                           <  1:951169/139‑1 (MQ=255)
                                                                                                      cGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTa                                             <  2:222146/118‑1 (MQ=255)
                                                                                                      cGAACGGGAAACTTCATAGGAGAAGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTa                                             >  1:222146/1‑118 (MQ=255)
                                                                                                                            aaGTCCACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCACGCTCACGCTCAAGATCCATGGAATCGAGAACCTgcg  >  2:290841/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                     
TTCGAGATCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGTTAAGCTGATAGGTTTCGCCGTCAGACGCTTTGTAGTCCAGCGTCACGCTTTGCGCTTTGATGGTAATGCCACGCTCACGCTCAAGATCCATGGAATCGAGAACCTGCG  >  NC_000913/2706750‑2707012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A