Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,421	T→C	P197P (CCT→CCC)	nadK →	NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,421	0	T	C	100.0%	129.1 / NA	36	P197P (CCT→CCC)	nadK	NAD kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/17); total (19/17)

GTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAAT  >  NC_000913/2751296‑2751555

gTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGa                                                                                                                                 >  2:409756/1‑133 (MQ=255)

gTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGa                                                                                                                                 <  1:409756/133‑1 (MQ=255)

    cgcATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCctctct                                                                                                                       >  1:448951/1‑139 (MQ=255)

                           tataTCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCa                                                                                                >  1:333749/1‑139 (MQ=255)

                              atCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGt                                                                                             <  2:259950/139‑1 (MQ=255)

                                 gacgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTcc                                                                                          <  1:747579/139‑1 (MQ=255)

                                   cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTgg                                                                                                      >  2:264864/1‑125 (MQ=255)

                                   cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTgg                                                                                                      <  1:264864/125‑1 (MQ=255)

                                   cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATg                                                                                              >  1:1089432/1‑133 (MQ=255)

                                   cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATg                                                                                              <  2:1089432/133‑1 (MQ=255)

                                   cgaGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCg                                                                                        >  2:711100/1‑139 (MQ=255)

                                                               gATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATg                                                                                              <  2:875391/105‑1 (MQ=255)

                                                               gATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATg                                                                                              >  1:875391/1‑105 (MQ=255)

                                                                   gACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGt                                                                                                     >  1:506656/1‑94 (MQ=255)

                                                                   gACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGt                                                                                                     <  2:506656/94‑1 (MQ=255)

                                                                     cTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATAc                                                                                   <  1:30775/110‑1 (MQ=255)

                                                                     cTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATAc                                                                                   >  2:30775/1‑110 (MQ=255)

                                                                        attattTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcagca                                                   <  1:490268/139‑1 (MQ=255)

                                                                              tCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAgcagcagca                                             >  2:477622/1‑139 (MQ=255)

                                                                                  cGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGAt                                         >  2:341291/1‑139 (MQ=255)

                                                                                                 ccGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGAc                                                                                                                                <  1:485339/37‑1 (MQ=255)

                                                                                                 ccGCCTATTCCCTCTCTGCAGGCGGTCCCATTCTGAc                                                                                                                                >  2:485339/1‑37 (MQ=255)

                                                                                                     cTATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                      >  1:691728/1‑139 (MQ=255)

                                                                                                      tATTCCCTCTCTGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATc                     >  1:914538/1‑139 (MQ=255)

                                                                                                            ctctctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTTGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTa               <  2:448951/139‑1 (MQ=255)

                                                                                                            ctctctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTa               <  1:477622/139‑1 (MQ=255)

                                                                                                            ctctctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTa               <  1:341291/139‑1 (MQ=255)

                                                                                                             tctctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa              <  2:914538/139‑1 (MQ=255)

                                                                                                                ctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa           >  2:581424/1‑139 (MQ=255)

                                                                                                                ctGCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa           >  2:90759/1‑139 (MQ=255)

                                                                                                                  gCAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAcc         <  1:581424/139‑1 (MQ=255)

                                                                                                                   cAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTgg                                                                                                      >  2:22378/1‑45 (MQ=255)

                                                                                                                   cAGGCGGTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTgg                                                                                                      <  1:22378/45‑1 (MQ=255)

                                                                                                                         gTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa              >  2:260211/1‑127 (MQ=255)

                                                                                                                         gTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa              <  1:260211/127‑1 (MQ=255)

                                                                                                                         gTCCCATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAAt  >  1:766953/1‑139 (MQ=255)

GTGGCGCATATGATTGAGTTCGAAGTGTATATCGACGAGATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAAT  >  NC_000913/2751296‑2751555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A