Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	826,119	C→T	378 (TAG→TAA)	ybhS ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	826,119	0	C	T	100.0%	187.9 / NA	58	378 (TAG→TAA)	ybhS	putative ABC transporter permease
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/30); total (28/30)

GCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACG  >  NC_000913/825987‑826254

gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATcc                                                                                                                                   >  1:860770/1‑139 (MQ=255)

gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATcc                                                                                                                                   >  2:1510230/1‑139 (MQ=255)

gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATcc                                                                                                                                   >  2:194423/1‑139 (MQ=255)

                 gATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGtttt                                                                                                                  >  2:52748/1‑139 (MQ=255)

                     aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                              >  1:1264499/1‑139 (MQ=255)

                           aTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTc                                                                                                        >  1:32056/1‑139 (MQ=255)

                            ttAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                       <  1:230992/139‑1 (MQ=255)

                              aGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAgg                                                                                                     <  2:860770/139‑1 (MQ=255)

                                cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGcc                                                                                                   >  1:453862/1‑139 (MQ=255)

                                 aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                       >  2:1645925/1‑134 (MQ=255)

                                 aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                       <  1:1645925/134‑1 (MQ=255)

                                 aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                  <  2:1155565/139‑1 (MQ=255)

                                 aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                  <  1:1520833/139‑1 (MQ=255)

                                 aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                  <  1:1293236/139‑1 (MQ=255)

                                     gTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                              <  1:52748/139‑1 (MQ=255)

                                           tCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCa                                                                                        <  2:1332822/139‑1 (MQ=255)

                                               aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGttt                                                                                                                        <  2:58114/103‑1 (MQ=255)

                                               aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGttt                                                                                                                        >  1:58114/1‑103 (MQ=255)

                                                    cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  2:1264499/139‑1 (MQ=255)

                                                    cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               >  2:921767/1‑139 (MQ=255)

                                                    cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGGTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               >  1:941985/1‑139 (MQ=255)

                                                                     cgcAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACg                                                              >  1:133788/1‑139 (MQ=255)

                                                                       cagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGtt                                                            <  1:98311/139‑1 (MQ=255)

                                                                              gACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTaccacc                                                     <  1:1228312/139‑1 (MQ=255)

                                                                                cTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                   <  2:898164/139‑1 (MQ=255)

                                                                                cTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                   <  1:1078595/139‑1 (MQ=255)

                                                                                  gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGt                                                                                                                     >  2:841431/1‑71 (MQ=255)

                                                                                  gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGt                                                                                                                     <  1:841431/71‑1 (MQ=255)

                                                                                  gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGca                                                 <  2:83305/139‑1 (MQ=255)

                                                                                   ctcctctTGGCGTTTAAACGTCCATCACCGATGACACCTAACCTTCCCATTAATCCAGCCGACGTTTGGTCTACAGCCACGTCAGGCCGATAAACTTCACCGCCGAAACGATCAAAAACAGCACGTTTACCACCAGcac                                                <  2:941985/136‑1 (MQ=255)

                                                                                   cAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  1:730879/139‑1 (MQ=255)

                                                                                     aCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                              <  1:1510230/139‑1 (MQ=255)

                                                                                                aTTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTc                                   >  2:1274129/1‑139 (MQ=255)

                                                                                                     cGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGc                              <  1:1274129/139‑1 (MQ=255)

                                                                                                     cGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGc                              <  2:32056/139‑1 (MQ=255)

                                                                                                          aTAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGa                         >  2:909499/1‑139 (MQ=255)

                                                                                                            aaGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                       <  2:453862/139‑1 (MQ=255)

                                                                                                                 aTGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGtttt                                                                                                                  >  1:775635/1‑43 (MQ=255)

                                                                                                                 aTGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGtttt                                                                                                                  <  2:775635/43‑1 (MQ=255)

                                                                                                                 aTGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCt                  >  1:282306/1‑139 (MQ=255)

                                                                                                                 aTGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCt                  >  2:154111/1‑139 (MQ=255)

                                                                                                                  tGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGaa                                        <  2:279339/116‑1 (MQ=255)

                                                                                                                  tGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGaa                                        >  1:279339/1‑116 (MQ=255)

                                                                                                                  tGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                       >  2:32184/1‑133 (MQ=255)

                                                                                                                  tGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                       <  1:32184/133‑1 (MQ=255)

                                                                                                                  tGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt                 >  1:1556580/1‑139 (MQ=255)

                                                                                                                      aCATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa             <  1:921767/139‑1 (MQ=255)

                                                                                                                          gCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                              >  2:847178/1‑38 (MQ=255)

                                                                                                                          gCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                              <  1:847178/38‑1 (MQ=255)

                                                                                                                          gCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGt         >  1:1324346/1‑139 (MQ=255)

                                                                                                                          gCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGt         >  2:1323266/1‑139 (MQ=255)

                                                                                                                              ttctCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                          <  1:1470715/54‑1 (MQ=255)

                                                                                                                              ttctCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                          >  2:1470715/1‑54 (MQ=255)

                                                                                                                                ctcCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                <  2:771236/78‑1 (MQ=255)

                                                                                                                                ctcCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                >  1:771236/1‑78 (MQ=255)

                                                                                                                                ctcCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGAc   >  2:1609898/1‑139 (MQ=255)

                                                                                                                                ctcCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGAc   >  1:756324/1‑139 (MQ=255)

                                                                                                                                 tcCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACg  <  2:665943/139‑1 (MQ=255)

GCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACG  >  NC_000913/825987‑826254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A