Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	828,477	C→T	V169I (GTT→ATT)	ybhF ←	putative ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	828,477	0	C	T	100.0%	131.6 / NA	41	V169I (GTT→ATT)	ybhF	putative ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/21); total (20/21)

CGCCTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAG  >  NC_000913/828342‑828602

cGCCTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAatgcc                                                                                                                            >  1:952297/1‑139 (MQ=255)

                   cacGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagc                                                                                                         <  2:941894/139‑1 (MQ=255)

                     cGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagcag                                                                                                       >  2:911782/1‑139 (MQ=255)

                                tGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCg                                                                                            >  1:579374/1‑139 (MQ=255)

                                  cTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          >  2:589576/1‑139 (MQ=255)

                                  cTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          >  2:198045/1‑139 (MQ=255)

                                  cTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          >  1:857818/1‑139 (MQ=255)

                                            tcgAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  1:198045/139‑1 (MQ=255)

                                            tcgAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  2:579374/139‑1 (MQ=255)

                                            tcgAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  1:911782/139‑1 (MQ=255)

                                            tcgAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  1:838274/139‑1 (MQ=255)

                                                gaTACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGt                                                                            <  2:99032/139‑1 (MQ=255)

                                                                  ggATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGttt                                                          >  1:828883/1‑139 (MQ=255)

                                                                   gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGtttt                                                         >  1:929004/1‑139 (MQ=255)

                                                                      tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagcagc                                                                                                      <  1:359908/91‑1 (MQ=255)

                                                                      tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagcagc                                                                                                      >  2:359908/1‑91 (MQ=255)

                                                                      tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc                                                                                     <  1:836738/108‑1 (MQ=255)

                                                                      tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc                                                                                     >  2:836738/1‑108 (MQ=255)

                                                                        aCATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGttt                                                    <  2:465622/139‑1 (MQ=255)

                                                                        aCATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGttt                                                    >  2:813590/1‑139 (MQ=255)

                                                                        aCATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGttt                                                    >  1:465622/1‑139 (MQ=255)

                                                                          aTCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCTAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCa                                                  <  1:813590/139‑1 (MQ=255)

                                                                                tCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccac                                                                                  >  1:520971/1‑101 (MQ=255)

                                                                                tCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccac                                                                                  <  2:520971/101‑1 (MQ=255)

                                                                                 cgcccgccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGt                                                                                                                  <  2:129428/68‑1 (MQ=255)

                                                                                 cgcccgccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGt                                                                                                                  >  1:129428/1‑68 (MQ=255)

                                                                                    ccgccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGga                                        >  1:541093/1‑139 (MQ=255)

                                                                                                    cATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAgg                        >  1:228934/1‑139 (MQ=255)

                                                                                                     aTCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          >  2:832980/1‑72 (MQ=255)

                                                                                                     aTCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt                                                                                          <  1:832980/72‑1 (MQ=255)

                                                                                                     aTCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                >  1:1050138/1‑82 (MQ=255)

                                                                                                     aTCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacca                                                                                <  2:1050138/82‑1 (MQ=255)

                                                                                                      tCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCg                      >  1:596998/1‑139 (MQ=255)

                                                                                                               gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa             <  2:268741/139‑1 (MQ=255)

                                                                                                               gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa             <  2:840861/139‑1 (MQ=255)

                                                                                                               gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa             <  2:857818/139‑1 (MQ=255)

                                                                                                                    cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGcc        <  1:412698/139‑1 (MQ=255)

                                                                                                                    cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGcc        >  2:412698/1‑139 (MQ=255)

                                                                                                                    cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGcc        <  2:929004/139‑1 (MQ=255)

                                                                                                                          gagaTAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAagag  <  1:639058/139‑1 (MQ=255)

                                                                                                                                 ggTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCg                                                                                            >  2:430882/1‑42 (MQ=255)

                                                                                                                                 ggTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCg                                                                                            <  1:430882/42‑1 (MQ=255)

CGCCTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAG  >  NC_000913/828342‑828602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A