Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
777 |
59.2 |
2955938 |
92.5% |
2734242 |
106.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
828,477 |
C→T |
V169I (GTT→ATT) |
ybhF ← |
putative ABC transporter ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 828,477 | 0 | C | T | 97.9%
| 152.7
/ NA
| 48 | V169I (GTT→ATT) | ybhF | putative ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (24/23); minor base G (0/1); total (24/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACT > NC_000913/828345‑828611
|
cTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAatgccgac > 2:1438635/1‑139 (MQ=255)
cTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAatgccgac < 2:360665/139‑1 (MQ=255)
atcaGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGtt > 2:1408685/1‑139 (MQ=255)
cacGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagc > 2:686361/1‑139 (MQ=255)
cGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGagcag > 1:777790/1‑139 (MQ=255)
tGCTCGGCTTCGACGAGATACGAGGTCCTCCATAGGATTAACATCCCTTCGCCCGCCAGCACATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAAGGCCGACGCCGGCTTCATCGAGCAGCAACACTTTCg < 1:287940/139‑1 (MQ=255)
cTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGt > 2:1441422/1‑139 (MQ=255)
cGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTc < 1:1408685/139‑1 (MQ=255)
cTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc < 1:668499/139‑1 (MQ=255)
cTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc > 2:668499/1‑139 (MQ=255)
cTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc < 1:1438635/139‑1 (MQ=255)
aTACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTa < 1:137529/139‑1 (MQ=255)
aTACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTa < 1:1441422/139‑1 (MQ=255)
cTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGAc < 2:777790/139‑1 (MQ=255)
tCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGAcc > 1:687759/1‑139 (MQ=255)
cAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTa < 1:1350920/126‑1 (MQ=255)
cAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTa > 2:1350920/1‑126 (MQ=255)
agGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGtt > 2:443662/1‑139 (MQ=255)
gATTAACATCCCTTCGCCGGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGtttt > 1:816316/1‑139 (MQ=255)
gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCg < 1:1345279/109‑1 (MQ=255)
gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCg > 2:1345279/1‑109 (MQ=255)
gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGtttt > 1:570351/1‑139 (MQ=255)
ttAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCcca < 1:1277509/111‑1 (MQ=255)
ttAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCcca > 2:1277509/1‑111 (MQ=255)
ttAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTg > 1:1247158/1‑139 (MQ=255)
tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacc < 2:302291/112‑1 (MQ=255)
tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccacc > 1:302291/1‑112 (MQ=255)
tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGt > 2:1134624/1‑139 (MQ=255)
ccTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGTaa > 2:780444/1‑86 (MQ=255)
ccTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCaa < 1:780444/86‑1 (MQ=255)
ccTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATcc < 1:1134624/139‑1 (MQ=255)
cTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCg < 1:1269884/98‑1 (MQ=255)
cTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCg > 2:1269884/1‑98 (MQ=255)
cgcccgccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCAcc > 2:759722/1‑139 (MQ=255)
cgcccgccAGCTCATGCACCATCTGCCACAG‑TCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCg < 1:677064/139‑1 (MQ=255)
gccAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGgaga < 1:759722/139‑1 (MQ=255)
cTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTg < 2:204180/139‑1 (MQ=255)
gCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCAt > 2:207243/1‑118 (MQ=255)
gCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCAt < 1:207243/118‑1 (MQ=255)
ccACAGTTCGCGCCATGAGATAGGGTCAATGCCGACGCCGGGTTCATCg > 1:1335040/1‑49 (MQ=255)
ccACAGTTCGCGCCATGAGATAGGGTCAATGCCGACGCCGGGTTCATCg < 2:1335040/49‑1 (MQ=255)
gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa < 1:443662/139‑1 (MQ=255)
gTTCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa < 2:687759/139‑1 (MQ=255)
ttCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc > 2:1477598/1‑66 (MQ=255)
ttCGCGCCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGcc < 1:1477598/66‑1 (MQ=255)
cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCaa > 1:1315743/1‑47 (MQ=255)
cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCaa < 2:1315743/47‑1 (MQ=255)
cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccac < 2:445737/65‑1 (MQ=255)
cgcCGTGAGATAGGGTCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCccac > 1:445737/1‑65 (MQ=255)
tCAATGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGGGTCCGGTAAACGGCCCAAGAGACGTAAACt > 2:1302128/1‑139 (MQ=255)
|
CTTCGTTCATCAGTAACACGTCACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACT > NC_000913/828345‑828611
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
