Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,385,339	C→T	P408L (CCG→CTG)	ycjX →	DUF463 family protein, puatative P‑loop NTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,385,339	0	C	T	100.0%	97.5 / NA	31	P408L (CCG→CTG)	ycjX	DUF463 family protein, puatative P‑loop NTPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/16); total (15/16)

AAAATGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATT  >  NC_000913/1385204‑1385466

aaaaTGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCt                                                                                                                              <  1:1264080/139‑1 (MQ=255)

aaaaTGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCt                                                                                                                              <  2:1157795/139‑1 (MQ=255)

   aTGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTc                                                                                                                             >  2:240449/1‑137 (MQ=255)

   aTGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTc                                                                                                                             <  1:240449/137‑1 (MQ=255)

          gTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTAt                                                                                                                    >  1:156769/1‑139 (MQ=255)

           ttCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATc                                                                                                                   <  2:1504412/139‑1 (MQ=255)

                             gACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCAc                                                                                                 >  2:616613/1‑139 (MQ=255)

                                        gCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGt                                                                                      <  2:74445/139‑1 (MQ=255)

                                           ggCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAg                                                                                   <  1:616613/139‑1 (MQ=255)

                                             cGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGc                                                                                 <  2:156769/139‑1 (MQ=255)

                                                cAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGtt                                                                              <  1:358535/139‑1 (MQ=255)

                                                                                gTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAt                                              >  1:816610/1‑139 (MQ=255)

                                                                                   aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCt                                                                                                                              >  2:1442119/1‑56 (MQ=255)

                                                                                   aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCt                                                                                                                              <  1:1442119/56‑1 (MQ=255)

                                                                                   aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCg                                                                                                    >  1:1552143/1‑82 (MQ=255)

                                                                                   aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCg                                                                                                    <  2:1552143/82‑1 (MQ=255)

                                                                                   aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTc                                           >  1:1367687/1‑139 (MQ=255)

                                                                                   aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTc                                           >  2:61178/1‑139 (MQ=255)

                                                                                     cGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGt                                         >  2:1350230/1‑139 (MQ=255)

                                                                                         gagaAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                     >  1:1140521/1‑139 (MQ=255)

                                                                                                      cgcTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGt                        >  2:1512236/1‑139 (MQ=255)

                                                                                                         tGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCACTTCGTCCGCAGGTGATGGATGTCGa                     <  2:1140521/139‑1 (MQ=255)

                                                                                                                        ttAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCa      <  2:1315374/139‑1 (MQ=255)

                                                                                                                         tAGCGATGGTGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatggatg                         <  1:1490050/119‑1 (MQ=255)

                                                                                                                         tAGCGATGGTGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatggatg                         >  2:1490050/1‑119 (MQ=255)

                                                                                                                          aGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGa                     >  1:601492/1‑122 (MQ=255)

                                                                                                                          aGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGa                     <  2:601492/122‑1 (MQ=255)

                                                                                                                          aGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCata    >  1:749347/1‑139 (MQ=255)

                                                                                                                            cGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATAtt  <  1:1512236/139‑1 (MQ=255)

                                                                                                                                 gcgcACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACt            <  1:363640/124‑1 (MQ=255)

                                                                                                                                 gcgcACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACt            >  2:363640/1‑124 (MQ=255)

AAAATGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATT  >  NC_000913/1385204‑1385466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A