Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I2 R1
|
760 |
67.6 |
3323148 |
92.7% |
3080558 |
109.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,416,884 |
A→G |
V168A (GTT→GCT) |
recE ← |
Rac prophage, exonuclease VIII, 5' to 3' specific dsDNA exonuclease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,416,884 | 0 | A | G | 96.5%
| 190.4
/ ‑2.7
| 57 | V168A (GTT→GCT) | recE | Rac prophage, exonuclease VIII, 5' to 3' specific dsDNA exonuclease |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (29/26); total (30/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 |
CTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTCCTGGCCAG > NC_000913/1416756‑1417019
|
cTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGc > 1:576547/1‑139 (MQ=255)
aaaaaaGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTaa > 2:401177/1‑139 (MQ=255)
aaaaaaGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTAGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGAGGGAAACAGCCAGGGCAGTaa > 2:1169447/1‑139 (MQ=255)
aaaaGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACa < 1:1607924/139‑1 (MQ=255)
aGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGcc < 2:1329842/139‑1 (MQ=255)
ttATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAg > 1:503675/1‑139 (MQ=255)
cAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttct < 2:806472/139‑1 (MQ=255)
cAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttct < 1:401177/139‑1 (MQ=255)
cAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGcc > 1:1235410/1‑139 (MQ=255)
ggTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGccc < 2:1050364/138‑1 (MQ=255)
ggTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCg < 2:1080961/139‑1 (MQ=255)
ggTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTAGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGccc > 1:1050364/1‑138 (MQ=255)
ggTTTGAATTACCAGCATTAGGGAAAACTTTGTCAGTGTCACGAACCGGTTTGTGGTGTTCGCGAATTTCTAGCGGGTCGAGCAGGCTGGCTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCGTGCGCCCg > 2:1602848/1‑139 (MQ=255)
gTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGa < 1:1169447/139‑1 (MQ=255)
gAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAagcagc > 2:842383/1‑101 (MQ=255)
gAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAagcagc < 1:842383/101‑1 (MQ=255)
gAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttcttc > 1:1455859/1‑128 (MQ=255)
gAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttcttc < 2:1455859/128‑1 (MQ=255)
ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGcc > 2:6791/1‑96 (MQ=255)
ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGcc < 1:6791/96‑1 (MQ=255)
ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATgtgt > 2:716606/1‑138 (MQ=255)
ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATgtgt < 1:716606/138‑1 (MQ=255)
ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTa > 2:1437342/1‑139 (MQ=255)
aGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTgg > 2:97205/1‑139 (MQ=255)
ggAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCaa > 1:613079/1‑123 (MQ=255)
ggAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCaa < 2:613079/123‑1 (MQ=255)
gAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttcttc < 1:767876/111‑1 (MQ=255)
gAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttcttc > 2:767876/1‑111 (MQ=255)
aaCTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAggg < 1:1085011/86‑1 (MQ=255)
aaCTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCg > 2:944453/1‑139 (MQ=255)
aaCTTTGACAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAggg > 2:1085011/1‑86 (MQ=255)
tGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTcgcg > 1:1564395/1‑139 (MQ=255)
tGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTcgcg > 1:1130792/1‑139 (MQ=255)
gTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAagcagc < 1:1355414/75‑1 (MQ=255)
gTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAagcagc > 2:1355414/1‑75 (MQ=255)
tgtCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAAc > 2:641678/1‑139 (MQ=255)
tCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGt > 1:99013/1‑139 (MQ=255)
gTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCgg > 2:293586/1‑139 (MQ=255)
tgGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGttttt < 2:1532143/139‑1 (MQ=255)
gAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCg < 1:1295898/139‑1 (MQ=255)
aGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGc > 2:1078665/1‑139 (MQ=255)
ttCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTg > 1:165966/1‑139 (MQ=255)
ttCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTg < 2:1130792/139‑1 (MQ=255)
tCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTcc < 1:1292561/104‑1 (MQ=255)
tCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTcc > 2:1292561/1‑104 (MQ=255)
ttCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGa < 1:97205/139‑1 (MQ=255)
tCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAg < 1:641678/139‑1 (MQ=255)
ccAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGc < 2:1564395/139‑1 (MQ=255)
cAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCa < 2:165966/139‑1 (MQ=255)
gggTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGcc < 1:1437342/139‑1 (MQ=255)
ggTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCa > 1:526582/1‑139 (MQ=255)
ggTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCa > 1:345036/1‑139 (MQ=255)
tCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAAc > 2:171010/1‑139 (MQ=255)
ggCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATggg < 2:45650/139‑1 (MQ=255)
ggCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATggg < 2:99013/139‑1 (MQ=255)
tGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAg < 1:1322102/54‑1 (MQ=255)
tGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAg > 2:1322102/1‑54 (MQ=255)
ttttGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTc < 2:345036/139‑1 (MQ=255)
tgGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCgtg > 2:621315/1‑107 (MQ=255)
tgGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCgtg < 1:621315/107‑1 (MQ=255)
ggAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTCCTGGCCa > 2:1398209/1‑139 (MQ=255)
gAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTCCTGGCCAg < 2:1311142/139‑1 (MQ=255)
|
CTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTCCTGGCCAG > NC_000913/1416756‑1417019
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
