Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,949,300	G→A	A408V (GCA→GTA)	aspS ←	aspartyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,300	0	G	A	100.0%	187.8 / NA	58	A408V (GCA→GTA)	aspS	aspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/31); total (27/31)

AGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATA  >  NC_000913/1949166‑1949434

aggtgaACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACAcc                                                                                                                                    >  1:47260/1‑139 (MQ=255)

  gtgaACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCa                                                                                                                                  >  1:767316/1‑139 (MQ=255)

              tGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGcaa                                                                                                                      <  2:1551333/139‑1 (MQ=255)

                 aTTGCCGTCAGGCCGCCTTGACCGTCGTCTTCAAACATCGGGATGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGcaacaa                                                                                                                   >  1:1655470/1‑139 (MQ=255)

                  ttGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAAt                                                                                                                  >  1:1143839/1‑139 (MQ=255)

                        tCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCtt                                                                                                            >  2:218693/1‑139 (MQ=255)

                             ccgccTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTtgttgt                                                                                                       <  1:186631/139‑1 (MQ=255)

                                       cgtcgtCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGTGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCgaa                                                                                             <  1:1376900/139‑1 (MQ=255)

                                            tcTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGaaaa                                                                                        <  1:1400096/139‑1 (MQ=255)

                                                 aaaCATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCata                                                                                   <  1:826383/139‑1 (MQ=255)

                                                     aTCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGc                                                                               >  1:229261/1‑139 (MQ=255)

                                                      tCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGcc                                                                              >  2:318309/1‑139 (MQ=255)

                                                      tCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGcc                                                                              >  1:1310546/1‑139 (MQ=255)

                                                       cGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCa                                                                             >  1:1478436/1‑139 (MQ=255)

                                                       cGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCa                                                                             >  2:922856/1‑139 (MQ=255)

                                                         ggAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATc                                                                           <  2:1143839/139‑1 (MQ=255)

                                                          gAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCt                                                                          >  2:1596804/1‑139 (MQ=255)

                                                            aGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTg                                                                        <  1:888061/139‑1 (MQ=255)

                                                             gTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGcaacaa                                                                                                                   >  2:1556388/1‑95 (MQ=255)

                                                             gTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGcaacaa                                                                                                                   <  1:1556388/95‑1 (MQ=255)

                                                              tCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTgcg                                                                      <  1:287355/139‑1 (MQ=255)

                                                              tCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTgcg                                                                      >  1:714293/1‑139 (MQ=255)

                                                                 aTAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  >  1:346926/1‑108 (MQ=255)

                                                                 aTAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  <  2:346926/108‑1 (MQ=255)

                                                                 aTAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCgg                                                                   <  2:47260/139‑1 (MQ=255)

                                                                      ccACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTa                                                              <  2:1346845/139‑1 (MQ=255)

                                                                       cacaGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTAc                                                             <  1:218693/139‑1 (MQ=255)

                                                                        acaGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGt                                                               >  2:1389597/1‑136 (MQ=255)

                                                                        acaGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGt                                                               <  1:1389597/136‑1 (MQ=255)

                                                                               tGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  >  2:245118/1‑94 (MQ=255)

                                                                               tGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  <  1:245118/94‑1 (MQ=255)

                                                                               tGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAgg                                                     >  1:195844/1‑139 (MQ=255)

                                                                               tGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAgg                                                     <  2:229261/139‑1 (MQ=255)

                                                                                gCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGa                                                    >  1:1055793/1‑139 (MQ=255)

                                                                                gCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAACTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTgcg                                                                      <  2:632479/121‑1 (MQ=255)

                                                                                gCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAACTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTgcg                                                                      >  1:632479/1‑121 (MQ=255)

                                                                                gCCCATTTGCTTTCGTCGGTCAGACCAAGGCCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  >  2:755421/1‑93 (MQ=255)

                                                                                gCCCATTTGCTTTCGTCGGTCAGACCAAGGCCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  <  1:755421/93‑1 (MQ=255)

                                                                                   cATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGt                                                 <  2:1478436/139‑1 (MQ=255)

                                                                                         cTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTGGTTGTCGGCACCGAAGGAAATCATATCGCCATCTTGCGCGGCAGTACGCTCCAGGATGTCTTCga                                           >  2:97745/1‑139 (MQ=255)

                                                                                               tcgGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGAttt                                     <  1:1596804/139‑1 (MQ=255)

                                                                                                            ggTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCg                                                                                               >  1:570656/1‑68 (MQ=255)

                                                                                                            ggTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCg                                                                                               <  2:570656/68‑1 (MQ=255)

                                                                                                            ggTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTa                                                              >  2:115392/1‑101 (MQ=255)

                                                                                                            ggTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTa                                                              <  1:115392/101‑1 (MQ=255)

                                                                                                                 ttACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCg                   >  2:1073603/1‑139 (MQ=255)

                                                                                                                       aCTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCg             >  2:1258200/1‑139 (MQ=255)

                                                                                                                       aCTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCg             >  2:453073/1‑139 (MQ=255)

                                                                                                                        cTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCgg            <  2:767316/139‑1 (MQ=255)

                                                                                                                         tttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGGACTTCGCTACCggg           <  2:1655470/139‑1 (MQ=255)

                                                                                                                         tttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCggg           <  1:922856/139‑1 (MQ=255)

                                                                                                                         tttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCggg           <  2:195844/139‑1 (MQ=255)

                                                                                                                          ttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGc          >  2:962005/1‑139 (MQ=255)

                                                                                                                          ttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGc          <  1:1073603/139‑1 (MQ=255)

                                                                                                                             aGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGt       <  2:957916/139‑1 (MQ=255)

                                                                                                                                 gcAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGAt   <  1:1403795/139‑1 (MQ=255)

                                                                                                                                  cAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGTGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACGTCGCTACCGGGCTGTTGATa  <  2:1322584/139‑1 (MQ=255)

                                                                                                                                  cAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATa  <  1:318309/139‑1 (MQ=255)

AGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATA  >  NC_000913/1949166‑1949434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A