Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,073,282	G→A	G582R (GGA→AGA)	flu →	CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,073,282	0	G	A	100.0%	944.0 / NA	283	G582R (GGA→AGA)	flu	CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (137/146); total (137/146)

Only 100 of 283 total aligned reads displayed.

GCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGAC  >  NC_000913/2073147‑2073417

gCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGAc                                                                                                                                      <  1:13029/139‑1 (MQ=255)

 cccTGAAGCTGACCGGCAGCACTTTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACa                                                                                                                                     >  1:1381404/1‑139 (MQ=255)

     gAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGAtt                                                                                                                                 <  2:1394335/139‑1 (MQ=255)

      aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGTCAGACAGATTc                                                                                                                                >  1:272581/1‑139 (MQ=255)

      aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTc                                                                                                                                >  1:638939/1‑139 (MQ=255)

        gCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCAt                                                                                                                              <  1:467290/139‑1 (MQ=255)

         cTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCAtt                                                                                                                             >  2:858507/1‑139 (MQ=255)

              cGGCAGCACTGTGCTGAACGGTGCCATTTACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCAcc                                                                                                                        <  1:992249/139‑1 (MQ=255)

              cGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCAcc                                                                                                                        <  2:603976/139‑1 (MQ=255)

              cGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCAcc                                                                                                                        <  1:324528/139‑1 (MQ=255)

              cGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCAcc                                                                                                                        <  1:1622821/139‑1 (MQ=255)

              cGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCAcc                                                                                                                        <  2:342754/139‑1 (MQ=255)

                   gcaCTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCAc                                                                                                                   <  1:895428/139‑1 (MQ=255)

                            tGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAgg                                                                                                          <  2:108896/139‑1 (MQ=255)

                            tGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAgg                                                                                                          <  1:1417246/139‑1 (MQ=255)

                                cGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAg                                                                                                      <  2:751907/139‑1 (MQ=255)

                                   tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCAc                                                                                                                   >  2:289255/1‑123 (MQ=255)

                                   tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCAc                                                                                                                   <  1:289255/123‑1 (MQ=255)

                                   tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTc                                                                                                   >  2:807142/1‑139 (MQ=255)

                                   tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTc                                                                                                   >  1:1275876/1‑139 (MQ=255)

                                   tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTc                                                                                                   >  1:96726/1‑139 (MQ=255)

                                   tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTc                                                                                                   >  1:596547/1‑139 (MQ=255)

                                        ttGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTAcc                                                                                              >  1:561146/1‑139 (MQ=255)

                                           aCCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                                                                           <  1:858507/139‑1 (MQ=255)

                                             cccACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCaa                                                                                         >  1:1520602/1‑139 (MQ=255)

                                                         aCTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGa                                                                             <  1:676814/139‑1 (MQ=255)

                                                           tctcGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGa                                                                             <  1:907028/137‑1 (MQ=255)

                                                           tctcGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGa                                                                             >  2:907028/1‑137 (MQ=255)

                                                              cGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAAc                                                                        >  2:49821/1‑139 (MQ=255)

                                                                ccTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCt                                                                      >  2:660541/1‑139 (MQ=255)

                                                                  tCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGa                                                                    <  2:561146/139‑1 (MQ=255)

                                                                         ccACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGa                                                               <  1:485750/137‑1 (MQ=255)

                                                                             cTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                                                                           <  1:788110/105‑1 (MQ=255)

                                                                               ggAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg                                                       <  2:371822/139‑1 (MQ=255)

                                                                                   tatCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCAcc                                                   <  1:882936/139‑1 (MQ=255)

                                                                                    atCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCa                                                  <  1:1207257/139‑1 (MQ=255)

                                                                                     tCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCAt                                                 >  2:163064/1‑139 (MQ=255)

                                                                                      ccccGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATc                                                >  1:408193/1‑139 (MQ=255)

                                                                                      ccccGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATc                                                >  2:1319983/1‑139 (MQ=255)

                                                                                          gATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGa                                                                    >  2:209467/1‑115 (MQ=255)

                                                                                           aTAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGcaca                                                                                                            <  2:1295244/74‑1 (MQ=255)

                                                                                           aTAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                                                                           <  1:821025/91‑1 (MQ=255)

                                                                                           aTAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGa                                                               <  2:1257725/119‑1 (MQ=255)

                                                                                           aTAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGa                                                               >  1:1257725/1‑119 (MQ=255)

                                                                                           aTAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCt                                           >  1:1356542/1‑139 (MQ=255)

                                                                                               cGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGca                                                                                                              >  2:899901/1‑68 (MQ=255)

                                                                                               cGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAgg                                                                                                          <  2:595026/72‑1 (MQ=255)

                                                                                               cGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCgg                                                                                            <  2:110849/86‑1 (MQ=255)

                                                                                               cGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                                                                           >  2:1600313/1‑87 (MQ=255)

                                                                                               cGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGaa                                                                                  >  1:1293125/1‑96 (MQ=255)

                                                                                               cGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGaa                                                                                  <  2:1293125/96‑1 (MQ=255)

                                                                                               cGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCgt                                       >  2:1471284/1‑139 (MQ=255)

                                                                                               cGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCgt                                       >  2:1330148/1‑139 (MQ=255)

                                                                                                gCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg                                                       >  2:1346887/1‑122 (MQ=255)

                                                                                                 ccACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCAt                                                 >  1:1643080/1‑127 (MQ=255)

                                                                                                   aCGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCAcc                                                                                                                        >  1:143820/1‑54 (MQ=255)

                                                                                                   aCGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCAcc                                                                                                                        <  2:143820/54‑1 (MQ=255)

                                                                                                    cGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCAtt                                                                                                                             >  1:1167861/1‑48 (MQ=255)

                                                                                                     ggTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGcc                                            <  2:1613661/128‑1 (MQ=255)

                                                                                                     ggTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGcc                                            >  1:1613661/1‑128 (MQ=255)

                                                                                                       tGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                                                                           >  2:1555452/1‑79 (MQ=255)

                                                                                                       tGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGccc                               >  2:33268/1‑139 (MQ=255)

                                                                                                       tGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGccc                               <  2:408193/139‑1 (MQ=255)

                                                                                                         cAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCgg                             >  2:694342/1‑139 (MQ=255)

                                                                                                          aGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCgg                                                                                            >  1:1184680/1‑75 (MQ=255)

                                                                                                          aGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg                                                       <  1:905903/112‑1 (MQ=255)

                                                                                                          aGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGa                            <  1:108892/139‑1 (MQ=255)

                                                                                                          aGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGa                            <  1:1330148/139‑1 (MQ=255)

                                                                                                          aGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGa                            >  1:662104/1‑139 (MQ=255)

                                                                                                           gTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGat                           >  2:4780/1‑139 (MQ=255)

                                                                                                            tCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGata                          <  1:1550492/139‑1 (MQ=255)

                                                                                                            tCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGata                          >  1:1658704/1‑139 (MQ=255)

                                                                                                            tCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCGGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGata                          <  2:1381404/139‑1 (MQ=255)

                                                                                                             cGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGa                                                                    >  1:386923/1‑96 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAATCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca                     >  2:951146/1‑139 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGa                                                                    <  2:824263/92‑1 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGa                                                                    >  1:824263/1‑92 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg                                                       >  1:1576437/1‑105 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca                     <  2:876457/139‑1 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca                     <  2:450854/139‑1 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca                     >  1:71708/1‑139 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca                     <  2:654661/139‑1 (MQ=255)

                                                                                                                 ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca                     <  2:1167183/139‑1 (MQ=255)

                                                                                                                   tggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGca                                                                                                              <  1:620559/48‑1 (MQ=255)

                                                                                                                     gATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGa                 <  2:625340/139‑1 (MQ=255)

                                                                                                                      aTGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGaa                <  1:747396/139‑1 (MQ=255)

                                                                                                                      aTGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGaa                <  1:937807/139‑1 (MQ=255)

                                                                                                                       tGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAAc               <  1:1039166/139‑1 (MQ=255)

                                                                                                                          ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                                                                           >  2:1646962/1‑60 (MQ=255)

                                                                                                                          ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGa                                                           >  1:450787/1‑92 (MQ=255)

                                                                                                                          ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTa                                    <  2:971985/115‑1 (MQ=255)

                                                                                                                            tCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                                                                           >  1:33925/1‑58 (MQ=255)

                                                                                                                             cAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCt         <  2:886753/139‑1 (MQ=255)

                                                                                                                               gCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGt                                                                                                     <  1:44125/45‑1 (MQ=255)

                                                                                                                               gCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGt                                                                                                     >  2:44125/1‑45 (MQ=255)

                                                                                                                                ccATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGTGTGTACGCCCGGata                          >  2:119874/1‑119 (MQ=255)

                                                                                                                                   tGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCgtg                                      <  1:1321310/104‑1 (MQ=255)

                                                                                                                                   tGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGa   >  2:1177481/1‑139 (MQ=255)

                                                                                                                                    gCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGAc  <  2:1520602/139‑1 (MQ=255)

                                                                                                                                    gCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGGGAAAAACCTGAACGGACAGAATGGCACCAGCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGAc  <  1:1593733/139‑1 (MQ=255)

GCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGAC  >  NC_000913/2073147‑2073417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A