Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I2 R1 760 67.6 3323148 92.7% 3080558 109.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,348,905 G→A D152N (GAC→AAC)  yrbL → Mg(2+)‑starvation‑stimulated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,348,9050GA100.0% 214.4 / NA 66D152N (GAC→AAC) yrbLMg(2+)‑starvation‑stimulated protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/33);  total (33/33)

AACTGCGCCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCGACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGCCCTGCAGAAAGATCTGC  >  NC_000913/3348777‑3349037
                                                                                                                                |                                                                                                                                    
aaCTGCGCCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTgg                                                                                                                            >  1:769979/1‑139 (MQ=255)
 aCTGCGCCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGt                                                                                                                           >  1:1634721/1‑139 (MQ=255)
  cTGCGCCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTg                                                                                                                          >  2:1387214/1‑139 (MQ=255)
     cgcCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGaaa                                                                                                                       >  1:1524657/1‑139 (MQ=255)
     cgcCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGaaa                                                                                                                       >  1:105150/1‑139 (MQ=255)
      gcCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAg                                                                                                                      <  2:769979/139‑1 (MQ=255)
      gcCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAg                                                                                                                      <  1:490016/139‑1 (MQ=255)
         aGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGcac                                                                                                                   <  1:499394/139‑1 (MQ=255)
          gCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGcaca                                                                                                                  <  2:416810/139‑1 (MQ=255)
            tACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACAtt                                                                                                                >  2:382377/1‑139 (MQ=255)
                        tGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGc                                                                                                    >  1:430493/1‑139 (MQ=255)
                            gCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGcac                                                                                                                   <  1:111119/120‑1 (MQ=255)
                            gCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGcac                                                                                                                   >  2:111119/1‑120 (MQ=255)
                                tATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGt                                                                                            <  2:518750/139‑1 (MQ=255)
                                 aTTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAg                                                                                                                      <  1:102115/112‑1 (MQ=255)
                                 aTTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAg                                                                                                                      >  2:102115/1‑112 (MQ=255)
                                 aTTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTc                                                                                           >  1:1472733/1‑139 (MQ=255)
                                    tGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGaa                                                                                        <  2:105150/139‑1 (MQ=255)
                                             aCCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGaa                                                                                                                        >  1:962256/1‑98 (MQ=255)
                                             aCCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGaa                                                                                                                        <  2:962256/98‑1 (MQ=255)
                                              ccGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGtgttgt                                                                              >  1:332821/1‑139 (MQ=255)
                                                  aTCGTTACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGc                                                                          <  2:430493/139‑1 (MQ=255)
                                                   tCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAgt                                                                                      <  2:207481/126‑1 (MQ=255)
                                                   tCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAgt                                                                                      >  1:207481/1‑126 (MQ=255)
                                                   tCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCg                                                                         >  2:727477/1‑139 (MQ=255)
                                                         cGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCa                                                                   <  1:465710/139‑1 (MQ=255)
                                                             gTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAagag                                                               <  1:727477/139‑1 (MQ=255)
                                                                gCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGc                                                                          >  1:870271/1‑125 (MQ=255)
                                                                gCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGc                                                                          <  2:870271/125‑1 (MQ=255)
                                                                      gCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATgg                                                      >  2:870568/1‑139 (MQ=255)
                                                                       ccGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGa                                                     <  2:1475670/139‑1 (MQ=255)
                                                                                    tctGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGc                                        >  2:1333343/1‑139 (MQ=255)
                                                                                      tGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGccc                                      >  1:592436/1‑139 (MQ=255)
                                                                                      tGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGccc                                      >  2:1407472/1‑139 (MQ=255)
                                                                                       gTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCa                                     >  1:853343/1‑139 (MQ=255)
                                                                                         cACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAAc                                   <  2:603001/139‑1 (MQ=255)
                                                                                           ccGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGa                                                                                                                         <  2:1060850/51‑1 (MQ=255)
                                                                                           ccGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGa                                                                                                                         >  1:1060850/1‑51 (MQ=255)
                                                                                           ccGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCg                                 >  2:650008/1‑139 (MQ=255)
                                                                                            cGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAacaaca                                                                                                                                >  2:198340/1‑43 (MQ=255)
                                                                                            cGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAacaaca                                                                                                                                <  1:198340/43‑1 (MQ=255)
                                                                                            cGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCgg                                >  1:21978/1‑139 (MQ=255)
                                                                                              cATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTAAGGAAACGATTTATCGCCCAACCGgcg                              <  2:1235091/139‑1 (MQ=255)
                                                                                                  aGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGgcgc                             >  1:156598/1‑136 (MQ=255)
                                                                                                  aGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGgcgc                             <  2:156598/136‑1 (MQ=255)
                                                                                                  aGTGAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTgg                          >  2:1401799/1‑139 (MQ=255)
                                                                                                     gAGTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAATCACATTGATTCCACTGGCGACCTGGTCGAAGAGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTa                       <  2:1076465/139‑1 (MQ=255)
                                                                                                       gTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAgtgg                                                                                    >  1:518343/1‑76 (MQ=255)
                                                                                                       gTCAGAGGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAgtgg                                                                                    <  2:518343/76‑1 (MQ=255)
                                                                                                          agagGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGtgttg                                                                               <  2:322061/78‑1 (MQ=255)
                                                                                                          agagGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGtgttg                                                                               >  1:322061/1‑78 (MQ=255)
                                                                                                          agagGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGcc                  <  2:592436/139‑1 (MQ=255)
                                                                                                          agagGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGcc                  <  2:1524657/139‑1 (MQ=255)
                                                                                                          agagGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGcc                  <  2:1472733/139‑1 (MQ=255)
                                                                                                          agagGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGcc                  <  1:1387214/139‑1 (MQ=255)
                                                                                                          agagGTTATCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGcc                  <  2:853343/139‑1 (MQ=255)
                                                                                                                 aTCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGgcg                              <  2:1288697/120‑1 (MQ=255)
                                                                                                                 aTCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGgcg                              >  1:1288697/1‑120 (MQ=255)
                                                                                                                  tCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGt                                                                                            <  1:224529/57‑1 (MQ=255)
                                                                                                                  tCCCGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGt                                                                                            >  2:224529/1‑57 (MQ=255)
                                                                                                                  tCCAGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGtgttgt                                                                              <  1:1031744/71‑1 (MQ=255)
                                                                                                                  acccGGTCGTCTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGtgttgt                                                                              >  2:1031744/2‑71 (MQ=255)
                                                                                                                       gtcgtcTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGCCCTGCAGAAAGATc     >  1:1386068/1‑139 (MQ=255)
                                                                                                                        tcgtcTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTa                                                                       >  2:312943/1‑72 (MQ=255)
                                                                                                                        tcgtcTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTa                                                                       <  1:312943/72‑1 (MQ=255)
                                                                                                                          gtcTGCAACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGCCCTGCAGAAAGATCTGc  <  2:21978/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                    
AACTGCGCCAGCTACTGAAACAGCTGAAGCGTTATTTGCAGGATAACCGTATCGTGACGATGTCGCTGAAGCCGCAGAATATCCTCTGTCACCGCATCAGTGAGTCAGAGGTTATCCCGGTCGTCTGCGACAACATTGGTGAAAGCACATTGATTCCACTGGCGACCTGGTCGAAGTGGTGTTGTCTGCGTAAGCAAGAGCGGTTATGGAAACGATTTATCGCCCAACCGGCGCTGGCTATTGCCCTGCAGAAAGATCTGC  >  NC_000913/3348777‑3349037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A