Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,610,477	A→G	H355R (CAC→CGC)	prfC →	peptide chain release factor RF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,610,477	0	A	G	100.0%	136.2 / NA	41	H355R (CAC→CGC)	prfC	peptide chain release factor RF‑3
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/20); total (21/20)

CCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAACTGTTCCGT  >  NC_000913/4610346‑4610610

ccgcgaccgcgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTgaa                                                                                                                                <  1:209313/139‑1 (MQ=255)

         cgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGt                                                                                                                         >  2:207312/1‑137 (MQ=255)

         cgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGt                                                                                                                         <  1:207312/137‑1 (MQ=255)

                          tggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTgaa                                                                                                                                <  2:236551/113‑1 (MQ=255)

                          tggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTgaa                                                                                                                                >  1:236551/1‑113 (MQ=255)

                               tCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGc                                                                                                 >  2:1118783/1‑139 (MQ=255)

                                 cGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGcac                                                                                               <  2:1453061/139‑1 (MQ=255)

                                  ggTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGAGCGTTCGCGCGGTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGcaca                                                                                              >  2:674017/1‑139 (MQ=255)

                                    tAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTACTACGCGCTGACCTTTATGGCGGATGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAAc                                                                                            <  1:1031542/139‑1 (MQ=255)

                                     aaaTATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAAcc                                                                                           >  1:866464/1‑139 (MQ=255)

                                       atatGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCAc                                                                                         >  1:87115/1‑139 (MQ=255)

                                         atGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGc                                                                                                     >  1:1412356/1‑125 (MQ=255)

                                         atGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGc                                                                                                     <  2:1412356/125‑1 (MQ=255)

                                         atGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACgg                                                                                       >  1:910355/1‑139 (MQ=255)

                                           gAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                                     >  1:311944/1‑139 (MQ=255)

                                           gAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                                     <  2:366620/139‑1 (MQ=255)

                                           gAAAAAGGCATCAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                                     <  1:1072845/139‑1 (MQ=255)

                                                           tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     <  2:910355/139‑1 (MQ=255)

                                                           tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     <  2:87115/139‑1 (MQ=255)

                                                           tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     <  2:404564/139‑1 (MQ=255)

                                                                   gTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     >  1:674226/1‑131 (MQ=255)

                                                                   gTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     <  2:674226/131‑1 (MQ=255)

                                                                            gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                                                >  1:737217/1‑127 (MQ=255)

                                                                            gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                                                <  2:737217/127‑1 (MQ=255)

                                                                            gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTg                                                    >  1:1182786/1‑139 (MQ=255)

                                                                             cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCa                                                                                          >  2:958592/1‑100 (MQ=255)

                                                                             cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCa                                                                                          <  1:958592/100‑1 (MQ=255)

                                                                             cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTga                                                   <  2:866464/139‑1 (MQ=255)

                                                                             cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTga                                                   >  2:1256947/1‑139 (MQ=255)

                                                                                   aTGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAgatgat                                             <  1:1256947/139‑1 (MQ=255)

                                                                                     gtggtgATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCCGCGACACCTTTACCCAGGGTGAGAtgatga                                           >  1:319427/1‑139 (MQ=255)

                                                                                       ggtgATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAg                                         >  1:847771/1‑139 (MQ=255)

                                                                                              tcCGACGCGCTGACCTTTATGGCGGGTGACCGTTcgcgcg                                                                                                                                     >  1:758146/1‑40 (MQ=255)

                                                                                                     cgcTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                                                >  2:393723/1‑102 (MQ=255)

                                                                                                     cgcTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                                                <  1:393723/102‑1 (MQ=255)

                                                                                                       cTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGa                         >  1:81061/1‑139 (MQ=255)

                                                                                                           ccTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACtt                     <  2:311944/139‑1 (MQ=255)

                                                                                                                   gCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAg             <  1:1118783/139‑1 (MQ=255)

                                                                                                                      ggTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACtt                     >  2:413739/1‑128 (MQ=255)

                                                                                                                      ggTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACtt                     <  1:413739/128‑1 (MQ=255)

                                                                                                                      ggTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAAc          >  1:1604833/1‑139 (MQ=255)

                                                                                                                              ttCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAACTGTTCcgt  >  1:98578/1‑139 (MQ=255)

CCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAACTGTTCCGT  >  NC_000913/4610346‑4610610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A