Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
766 |
55.6 |
2631458 |
94.7% |
2491990 |
109.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
915,730 |
T→C |
K106K (AAA→AAG) |
aqpZ ← |
aquaporin Z |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 915,730 | 0 | T | C | 100.0%
| 193.4
/ NA
| 53 | K106K (AAA→AAG) | aqpZ | aquaporin Z |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/26); total (27/26) |
CGTGGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTTTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCG > NC_000913/915597‑915854
|
cGTGGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTAcc > 1:70918/1‑139 (MQ=255)
tGGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCAc > 2:175049/1‑139 (MQ=255)
aTCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg < 2:1232073/139‑1 (MQ=255)
aCCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa < 1:30715/139‑1 (MQ=255)
aCAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTaa > 1:1239340/1‑139 (MQ=255)
cAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTaaa < 2:70918/139‑1 (MQ=255)
ggAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATa > 2:192135/1‑139 (MQ=255)
aCCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATAcagc > 1:39180/1‑139 (MQ=255)
gCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAgcg < 1:479973/139‑1 (MQ=255)
cAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCt < 1:982766/139‑1 (MQ=255)
ttCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATa > 1:1180268/1‑139 (MQ=255)
cAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATa > 2:565614/1‑139 (MQ=255)
cAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAAtt > 2:945398/1‑110 (MQ=255)
cAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAAtt < 1:945398/110‑1 (MQ=255)
cAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATAcagcag > 1:363667/1‑121 (MQ=255)
cAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATAcagcag < 2:363667/121‑1 (MQ=255)
aCTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTaaa < 2:974770/111‑1 (MQ=255)
aCTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTaaa > 1:974770/1‑111 (MQ=255)
aGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACaa > 2:1005254/1‑139 (MQ=255)
aGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCATCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACaa > 2:1229923/1‑139 (MQ=255)
aaaGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGc < 2:1239340/139‑1 (MQ=255)
tAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGc > 2:180155/1‑139 (MQ=255)
ccGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCaa > 1:1294835/1‑139 (MQ=255)
ccGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCaa > 2:91529/1‑139 (MQ=255)
ccGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCaa < 2:1180268/139‑1 (MQ=255)
ccTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGa > 1:1243582/1‑139 (MQ=255)
cTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGAc < 1:192135/139‑1 (MQ=255)
tG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg < 2:983230/71‑1 (MQ=255)
tG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg > 1:983230/1‑71 (MQ=255)
tG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg < 1:953193/117‑1 (MQ=255)
tG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg > 2:953193/1‑117 (MQ=255)
ttAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGc < 1:91529/139‑1 (MQ=255)
aGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCa < 1:1005254/139‑1 (MQ=255)
aGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCa < 1:180155/139‑1 (MQ=255)
aGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCACCGACAACCTGGACAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCa > 2:1078161/1‑139 (MQ=255)
gCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGccc > 2:416360/1‑139 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc > 1:746326/1‑64 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc < 2:746326/64‑1 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa < 2:475790/139‑1 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa > 1:826569/1‑139 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa < 1:401437/139‑1 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa < 2:1294835/139‑1 (MQ=255)
aaaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACg > 2:391647/1‑97 (MQ=255)
aaaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACg < 1:391647/97‑1 (MQ=255)
aaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg < 2:482557/40‑1 (MQ=255)
aaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg > 1:482557/1‑40 (MQ=255)
aaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATa < 2:1243582/139‑1 (MQ=255)
aaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATa > 2:28708/1‑139 (MQ=255)
gctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGAc > 2:287123/1‑75 (MQ=255)
gctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGAc < 1:287123/75‑1 (MQ=255)
gctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTggg > 2:620106/1‑83 (MQ=255)
gctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTggg < 1:620106/83‑1 (MQ=255)
gcgTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGAccg < 1:175049/139‑1 (MQ=255)
|
CGTGGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTG‑‑GTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTTTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCG > NC_000913/915597‑915854
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
