Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
915,730 |
T→C |
K106K (AAA→AAG) |
aqpZ ← |
aquaporin Z |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 915,730 | 0 | T | C | 100.0%
| 149.8
/ NA
| 43 | K106K (AAA→AAG) | aqpZ | aquaporin Z |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/25); total (18/25) |
GGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTTTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCG > NC_000913/915600‑915854
|
ggATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt < 2:174059/139‑1 (MQ=255)
aTCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGTGTCAAAACCCGTCTTACCACTgg < 1:137614/139‑1 (MQ=255)
aTCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg < 1:191731/139‑1 (MQ=255)
aCCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa < 1:47073/139‑1 (MQ=255)
cAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTaaa < 1:105208/139‑1 (MQ=255)
tGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACagcagc < 2:741674/139‑1 (MQ=255)
tACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa > 2:241073/1‑116 (MQ=255)
tACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa < 1:241073/116‑1 (MQ=255)
tCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATAc < 2:1127036/139‑1 (MQ=255)
aCTACCAGCGCGGAAAGAATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATAccgc > 2:530087/1‑139 (MQ=255)
tACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCTGCGCCGCTGCAACAATAccgccg < 1:530087/139‑1 (MQ=255)
aCCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc > 2:275604/1‑122 (MQ=255)
aCCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc < 1:275604/122‑1 (MQ=255)
aGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACaa > 2:1036326/1‑139 (MQ=255)
cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt > 1:1180555/1‑93 (MQ=255)
cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt < 2:1180555/93‑1 (MQ=255)
cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg > 1:261293/1‑95 (MQ=255)
cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg < 2:261293/95‑1 (MQ=255)
cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc > 1:724662/1‑117 (MQ=255)
cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc < 2:724662/117‑1 (MQ=255)
gcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCt > 1:1241908/1‑139 (MQ=255)
cgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAgcgc > 2:626897/1‑112 (MQ=255)
cgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAgcgc < 1:626897/112‑1 (MQ=255)
gAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTggg < 1:1036326/139‑1 (MQ=255)
tGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc < 2:295928/106‑1 (MQ=255)
tGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc > 1:295928/1‑106 (MQ=255)
ccTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCAc < 2:799080/70‑1 (MQ=255)
ccTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCAc > 1:799080/1‑70 (MQ=255)
ccTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc > 1:263775/1‑95 (MQ=255)
ccTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc < 2:263775/95‑1 (MQ=255)
ttAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGc > 1:1058117/1‑139 (MQ=255)
aGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGcc > 2:1005951/1‑135 (MQ=255)
aGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGcc < 1:1005951/135‑1 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg < 2:1075205/88‑1 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg > 1:1075205/1‑88 (MQ=255)
cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa < 1:1303316/139‑1 (MQ=255)
aaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa < 2:1343020/43‑1 (MQ=255)
aaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa > 1:1343020/1‑43 (MQ=255)
aaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa < 2:1165905/136‑1 (MQ=255)
aaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa > 1:1165905/1‑136 (MQ=255)
ctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc > 1:668243/1‑56 (MQ=255)
ctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc < 2:668243/56‑1 (MQ=255)
gcgTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGAccg < 1:1293288/139‑1 (MQ=255)
|
GGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTTTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCG > NC_000913/915600‑915854
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
