Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 766 55.6 2631458 94.7% 2491990 109.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,351,196 (G)7→8 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,1891.G100.0% 199.2 / NA 55intergenic (‑150/+218)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (30/25);  total (30/25)

ATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351060‑1351319
                                                                                                                                  |                                                                                                                                  
aTGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑ggggggggc                                                                                                                           >  2:1244924/1‑137 (MQ=255)
           gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCt                                                                                                                 <  1:1278826/139‑1 (MQ=255)
           gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCt                                                                                                                 <  1:1244924/139‑1 (MQ=255)
            ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTc                                                                                                                >  1:75059/1‑139 (MQ=255)
            ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTc                                                                                                                >  1:579752/1‑139 (MQ=255)
                    tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        >  1:1026628/1‑139 (MQ=255)
                       ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCct                                                                                                     <  2:75059/139‑1 (MQ=255)
                                             cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCTTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAc                                                                               >  2:330695/1‑139 (MQ=255)
                                                tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCGCCAGAGGGGCGAGGGGACCGt                                                                            >  1:772890/1‑139 (MQ=255)
                                                tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                            >  1:1083803/1‑139 (MQ=255)
                                                 aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCTAGAGGGGCGAGGGGACCGTa                                                                           <  2:772890/139‑1 (MQ=255)
                                                  cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCa                                                                                                                                     <  1:1272755/80‑1 (MQ=255)
                                                  cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCa                                                                                                                                     >  2:1272755/1‑80 (MQ=255)
                                                  cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAcc                                                                                                       >  1:1229562/1‑110 (MQ=255)
                                                  cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAcc                                                                                                       <  2:1229562/110‑1 (MQ=255)
                                                     aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGAACGAAGCAGGCTGCGGCAGGGGGGGGCGCCTTTCCCCTCACCCTAACCCCCTCCCCAGAGGGGCGAGGCGACCGAATtga                                                                       >  2:71110/1‑138 (MQ=38)
                                                        gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                <  2:964902/127‑1 (MQ=255)
                                                        gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                >  1:964902/1‑127 (MQ=255)
                                                        gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                    >  2:1257948/1‑139 (MQ=255)
                                                          tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCt                                                                                                                 <  1:359690/92‑1 (MQ=255)
                                                          tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCt                                                                                                                 >  2:359690/1‑92 (MQ=255)
                                                              cGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                              >  1:864668/1‑139 (MQ=255)
                                                                 ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                           <  2:719007/123‑1 (MQ=255)
                                                                 ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                           >  1:719007/1‑123 (MQ=255)
                                                                   attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        <  2:1066555/92‑1 (MQ=255)
                                                                   attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        >  1:1066555/1‑92 (MQ=255)
                                                                   attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCct                                                                                                     >  1:1113015/1‑95 (MQ=255)
                                                                   attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCct                                                                                                     <  2:1113015/95‑1 (MQ=255)
                                                                         aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑ggggggggcg                                                                                                                          >  2:454390/1‑64 (MQ=255)
                                                                         aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑ggggggggcg                                                                                                                          <  1:454390/67‑4 (MQ=255)
                                                                              ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaacaa                                              >  2:686612/1‑139 (MQ=255)
                                                                              ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaacaa                                              >  1:913098/1‑139 (MQ=255)
                                                                               cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  2:1083803/139‑1 (MQ=255)
                                                                                        aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc                                                                                       <  2:689717/88‑1 (MQ=255)
                                                                                        aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc                                                                                       >  1:689717/1‑88 (MQ=255)
                                                                                            gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                      >  2:671259/1‑101 (MQ=255)
                                                                                            gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                      <  1:671259/101‑1 (MQ=255)
                                                                                                  gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                          >  1:1101218/1‑139 (MQ=255)
                                                                                                     gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        <  1:1110991/58‑1 (MQ=39)
                                                                                                     gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        <  1:881911/58‑1 (MQ=39)
                                                                                                     gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        >  2:1110991/1‑58 (MQ=39)
                                                                                                     gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAAc                                                                                                        >  2:881911/1‑58 (MQ=39)
                                                                                                     gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGca                                                  >  1:247077/1‑112 (MQ=255)
                                                                                                     gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGca                                                  <  2:247077/112‑1 (MQ=255)
                                                                                                      ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGGGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAccc                      >  2:462899/1‑139 (MQ=255)
                                                                                                          gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTcccc                                                                                                                  >  1:265171/1‑43 (MQ=255)
                                                                                                          gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTcccc                                                                                                                  <  2:265171/43‑1 (MQ=255)
                                                                                                          gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                   <  2:246318/74‑1 (MQ=255)
                                                                                                          gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                   >  1:246318/1‑74 (MQ=255)
                                                                                                               aCCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     <  1:1133350/67‑1 (MQ=255)
                                                                                                               aCCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                     >  2:1133350/1‑67 (MQ=255)
                                                                                                               aCCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc             <  2:1101218/139‑1 (MQ=255)
                                                                                                                    aGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATgg        >  1:614943/1‑139 (MQ=255)
                                                                                                                         gCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTggg   >  2:1186888/1‑139 (MQ=255)
                                                                                                                          cTGCGGCAGGGGGGGGCGCTTTTCCCTTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:330695/139‑1 (MQ=255)
                                                                                                                          cTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:864668/139‑1 (MQ=255)
                                                                                                                          cTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:913098/139‑1 (MQ=255)
                                                                                                                          cTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:686612/139‑1 (MQ=255)
                                                                                                                            gCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             >  1:159696/1‑94 (MQ=255)
                                                                                                                            gCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                             <  2:159696/94‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                  
ATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351060‑1351319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A