Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 766 55.6 2631458 94.7% 2491990 109.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,799,883 T→C K39K (AAA→AAG)  rpmI ← 50S ribosomal subunit protein L35

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,8830TC100.0% 167.3 / NA 47K39K (AAA→AAG) rpmI50S ribosomal subunit protein L35
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/21);  total (26/21)

ATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCG  >  NC_000913/1799750‑1800018
                                                                                                                                     |                                                                                                                                       
atGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGt                                                                                                                                    <  2:749080/139‑1 (MQ=255)
     ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCtt                                                                                                                               >  2:749171/1‑139 (MQ=255)
        tcttGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCttttt                                                                                                                            <  2:336511/136‑1 (MQ=255)
          cTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTg                                                                                                                          >  2:584306/1‑139 (MQ=255)
             tATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTc                                                                                                                       >  1:1310599/1‑139 (MQ=255)
              aTCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCa                                                                                                                      <  2:1150884/139‑1 (MQ=255)
                       cTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGTCTTTGGAAACCATGGCTTTAGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGAc                                                                                                             <  1:166322/139‑1 (MQ=255)
                       cTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGAc                                                                                                             >  1:1137097/1‑139 (MQ=255)
                       cTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGAc                                                                                                             >  2:244131/1‑139 (MQ=255)
                         aaTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAtg                                                                                                                 >  1:1210882/1‑133 (MQ=255)
                         aaTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAtg                                                                                                                 <  2:1210882/133‑1 (MQ=255)
                          aTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCa                                                                                                          >  1:1246934/1‑139 (MQ=255)
                          aTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCa                                                                                                          >  1:230249/1‑139 (MQ=255)
                               aaaGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTa                                                                                                     >  2:580021/1‑139 (MQ=255)
                               aaaGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTa                                                                                                     <  2:230249/139‑1 (MQ=255)
                                             tAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGt                                                                                                                        <  1:24006/106‑1 (MQ=255)
                                             tAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGt                                                                                                                        >  2:24006/1‑106 (MQ=255)
                                                  gCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAac                                                                                  >  2:43868/1‑139 (MQ=255)
                                                            aCGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTAcc                                                                        >  1:954453/1‑139 (MQ=255)
                                                              ggcaggcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCgg                                                                      <  1:1269184/139‑1 (MQ=255)
                                                                   gcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGttttt                                                                 >  1:604130/1‑139 (MQ=255)
                                                                   gcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGttttt                                                                 >  1:218204/1‑139 (MQ=255)
                                                                                     cAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGt                                                                                                                        <  1:929088/66‑1 (MQ=255)
                                                                                     cAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGt                                                                                                                        >  2:929088/1‑66 (MQ=255)
                                                                                     cAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAgcagca                                               <  1:244131/139‑1 (MQ=255)
                                                                                      aGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCAc                                              <  1:580021/139‑1 (MQ=255)
                                                                                        aTCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACcg                                            >  2:1307149/1‑139 (MQ=255)
                                                                                          cGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACcgcg                                          <  1:43868/139‑1 (MQ=255)
                                                                                              tttGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACg                                      >  2:579240/1‑139 (MQ=255)
                                                                                                tGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGc                                                                                                           >  1:521225/1‑68 (MQ=255)
                                                                                                tGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGc                                                                                                           <  2:521225/68‑1 (MQ=255)
                                                                                                tGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgct                                                                                              >  2:1153950/1‑81 (MQ=255)
                                                                                                tGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgct                                                                                              <  1:1153950/81‑1 (MQ=255)
                                                                                                tGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGt                                    >  2:663167/1‑139 (MQ=255)
                                                                                                       cATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTa                                                                                                     >  1:315054/1‑67 (MQ=255)
                                                                                                       cATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTa                                                                                                     <  2:315054/67‑1 (MQ=255)
                                                                                                         tGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAt                                                                                                                  >  2:499027/1‑52 (MQ=255)
                                                                                                         tGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAt                                                                                                                  <  1:499027/52‑1 (MQ=255)
                                                                                                         tGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAAtttt                           >  1:648167/1‑139 (MQ=255)
                                                                                                            cTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         >  1:534168/1‑106 (MQ=255)
                                                                                                            cTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  2:1100721/106‑1 (MQ=255)
                                                                                                            cTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  2:534168/106‑1 (MQ=255)
                                                                                                            cTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         >  1:1100721/1‑106 (MQ=255)
                                                                                                             tttCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGc                       <  2:218204/139‑1 (MQ=255)
                                                                                                             tttCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGc                       <  2:1310599/139‑1 (MQ=255)
                                                                                                                 ggACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCATGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                        <  2:92981/118‑1 (MQ=255)
                                                                                                                 ggACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCATGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                        >  1:92981/1‑118 (MQ=255)
                                                                                                                                ttacgcttGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACtt    >  1:1010746/1‑139 (MQ=255)
                                                                                                                                 tacgcttGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  1:1094363/85‑1 (MQ=255)
                                                                                                                                 tacgcttGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         >  2:1094363/1‑85 (MQ=255)
                                                                                                                                  acgcttGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCg  <  2:1010746/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
ATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCG  >  NC_000913/1799750‑1800018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A