Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,799,883	T→C	K39K (AAA→AAG)	rpmI ←	50S ribosomal subunit protein L35

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,799,883	0	T	C	100.0%	65.4 / NA	21	K39K (AAA→AAG)	rpmI	50S ribosomal subunit protein L35
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/9); total (12/9)

CTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTC  >  NC_000913/1799760‑1800017

cTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTg                                                                                                                         >  1:316450/1‑139 (MQ=255)

  gTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGt                                                                                                                       <  1:372447/139‑1 (MQ=255)

   tATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTCGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTc                                                                                                                      >  2:418990/1‑139 (MQ=255)

                aTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCa                                                                                                         >  1:298742/1‑139 (MQ=255)

                     aaaGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTa                                                                                                    >  1:192691/1‑139 (MQ=255)

                      aaGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAg                                                                                                   <  2:192691/139‑1 (MQ=255)

                                           tATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCa                                                                                                                     <  1:21331/100‑1 (MQ=255)

                                           tATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCa                                                                                                                     >  2:21331/1‑100 (MQ=255)

                                               cGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttg                                                                                           <  2:154717/122‑1 (MQ=255)

                                               cGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttg                                                                                           >  1:154717/1‑122 (MQ=255)

                                                 tACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTAc                                                                        >  2:481152/1‑139 (MQ=255)

                                                   cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCg                                                                      >  1:805075/1‑139 (MQ=255)

                                                                       ggCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAtg                                                                                                                <  1:268757/77‑1 (MQ=255)

                                                                       ggCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAtg                                                                                                                >  2:268757/1‑77 (MQ=255)

                                                                             gATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                        <  1:523821/127‑1 (MQ=255)

                                                                             gATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                        >  2:523821/1‑127 (MQ=255)

                                                                                            ccATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAAt                             >  2:128497/1‑139 (MQ=255)

                                                                                                ggCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAAttttt                         >  1:232583/1‑139 (MQ=255)

                                                                                                   tttCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGc                      <  2:316450/139‑1 (MQ=255)

                                                                                                     tCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCAt                    <  2:805075/139‑1 (MQ=255)

                                                                                                         aCGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTta                <  1:516657/139‑1 (MQ=255)

                                                                                                                       tacgcttGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTc  >  1:268064/1‑139 (MQ=255)

CTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTC  >  NC_000913/1799760‑1800017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A