Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 766 55.6 2631458 94.7% 2491990 109.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,189,594 C→T A439A (GCG→GCA)  yehB ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,189,5940CT100.0% 194.3 / NA 60A439A (GCG→GCA) yehBputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/30);  total (30/30)

GTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCG  >  NC_000913/2189480‑2189728
                                                                                                                  |                                                                                                                                      
gTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGtt                                                                                                                >  2:250584/1‑139 (MQ=255)
gTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGtt                                                                                                                <  1:124665/139‑1 (MQ=255)
     aatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCt                                                                                                           <  2:536260/139‑1 (MQ=255)
          gCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACaa                                                                                                      >  1:428423/1‑139 (MQ=255)
                   tAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTtgttgt                                                                                             >  2:214944/1‑139 (MQ=255)
                         tCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                       >  2:537165/1‑139 (MQ=255)
                          cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGc                                                                                         <  1:666441/136‑1 (MQ=255)
                          cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGc                                                                                         >  2:666441/1‑136 (MQ=255)
                          cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                      >  1:505068/1‑139 (MQ=255)
                          cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                      >  2:447396/1‑139 (MQ=255)
                          cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                      >  2:1071887/1‑139 (MQ=255)
                          cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                      >  2:820713/1‑139 (MQ=255)
                          cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                      >  1:1326/1‑139 (MQ=255)
                          cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                      >  1:1287287/1‑139 (MQ=255)
                               tcatcaCGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGAt                                                                                 <  1:405144/139‑1 (MQ=255)
                                       gCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                         <  1:74604/139‑1 (MQ=255)
                                       gCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                         <  2:839831/139‑1 (MQ=255)
                                          aTAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCg                                                                      <  1:820713/139‑1 (MQ=255)
                                                    ttATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt                                                            <  1:866696/139‑1 (MQ=255)
                                                     tATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTc                                                                                                                      >  2:1209445/1‑80 (MQ=255)
                                                     tATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTc                                                                                                                      <  1:1209445/80‑1 (MQ=255)
                                                      aTTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGAGCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                          >  1:820367/1‑139 (MQ=255)
                                                      aTTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                          >  2:458072/1‑139 (MQ=255)
                                                      aTTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                          >  2:700572/1‑139 (MQ=255)
                                                        tGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAAt                                                                                                    <  2:357273/95‑1 (MQ=255)
                                                        tGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAAt                                                                                                    >  1:357273/1‑95 (MQ=255)
                                                           ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAAt                                                                                                    >  2:858719/1‑92 (MQ=255)
                                                           ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAAt                                                                                                    <  1:858719/92‑1 (MQ=255)
                                                           ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                     <  1:1147087/139‑1 (MQ=255)
                                                             gCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGt                                                   <  2:1213330/139‑1 (MQ=255)
                                                              cccAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTc                                                  <  2:1326/139‑1 (MQ=255)
                                                               ccAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCt                                                 <  1:214944/139‑1 (MQ=255)
                                                                      tGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                       <  1:16795/126‑1 (MQ=255)
                                                                      tGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                       >  2:16795/1‑126 (MQ=255)
                                                                        aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                       <  1:216211/92‑1 (MQ=255)
                                                                        aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                       >  2:216211/1‑92 (MQ=255)
                                                                        aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGAt                                                                                 >  1:384313/1‑98 (MQ=255)
                                                                        aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGAt                                                                                 <  2:384313/98‑1 (MQ=255)
                                                                        aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTa                                        >  2:1020330/1‑139 (MQ=255)
                                                                        aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTa                                        >  2:679954/1‑139 (MQ=255)
                                                                        aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTa                                        >  2:413501/1‑139 (MQ=255)
                                                                             tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAA‑TTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGtt                                             <  1:53506/128‑1 (MQ=255)
                                                                             tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAA‑TTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGtt                                             >  2:53506/1‑128 (MQ=255)
                                                                                     cGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAaca                                                               >  2:385874/1‑103 (MQ=255)
                                                                                     cGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAaca                                                               <  1:385874/103‑1 (MQ=255)
                                                                                       gTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGTCTTAGTGGCa                         <  2:1169947/139‑1 (MQ=255)
                                                                                             aCGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACg                   <  1:447396/139‑1 (MQ=255)
                                                                                               gcgACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGtt                                                                                                                <  2:301820/44‑1 (MQ=255)
                                                                                               gcgACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGtt                                                                                                                >  1:301820/1‑44 (MQ=255)
                                                                                                cgacgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAgg                                                                                          >  2:53564/1‑65 (MQ=255)
                                                                                                cgacgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAgg                                                                                          <  1:53564/65‑1 (MQ=255)
                                                                                                  acgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAAt              >  1:488489/1‑139 (MQ=255)
                                                                                                      aTAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGct          <  2:820367/139‑2 (MQ=255)
                                                                                                         aCGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGa                                   <  2:1242934/111‑1 (MQ=255)
                                                                                                         aCGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGa                                   >  1:1242934/1‑111 (MQ=255)
                                                                                                           gCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAt     <  2:1312134/139‑1 (MQ=255)
                                                                                                           gCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAt     <  1:700572/139‑1 (MQ=255)
                                                                                                             cAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGc   <  1:250584/139‑1 (MQ=255)
                                                                                                             cAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGc   >  2:577712/1‑139 (MQ=255)
                                                                                                              aGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCg  <  2:428423/139‑1 (MQ=255)
                                                                                                                  |                                                                                                                                      
GTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCG  >  NC_000913/2189480‑2189728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A