Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I1 R1 776 60.0 3074904 90.1% 2770488 104.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,195,459 T→C V387A (GTT→GCT)  metG → methionyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,195,4590TC100.0% 197.5 / NA 56V387A (GTT→GCT) metGmethionyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

GCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGA  >  NC_000913/2195329‑2195591
                                                                                                                                  |                                                                                                                                    
gCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAg                                                                                                                              >  1:750734/1‑139 (MQ=255)
 ccAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGt                                                                                                                             >  1:1184425/1‑139 (MQ=255)
   aGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           <  2:750734/139‑1 (MQ=255)
   aGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           >  1:1086117/1‑139 (MQ=255)
                        gACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATg                                                                                                      <  2:1503167/139‑1 (MQ=255)
                                   ccTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCg                                                                                                           >  2:77007/1‑123 (MQ=255)
                                   ccTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCg                                                                                                           <  1:77007/123‑1 (MQ=255)
                                           actactacACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           >  1:138224/1‑99 (MQ=255)
                                           actactacACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           <  2:138224/99‑1 (MQ=255)
                                                 acacTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGa                                                                             >  2:646662/1‑139 (MQ=255)
                                                     tGCGAAACTCTCTTCGCGCATTGATGATGTCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATCCCGATATCGCTATCAAAGTGGTTAACCTGGCCTCCGGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGc                                                                         >  2:377334/1‑139 (MQ=255)
                                                     tGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 >  1:1534719/1‑99 (MQ=255)
                                                     tGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 <  2:1534719/99‑1 (MQ=255)
                                                     tGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGc                                                                         >  1:1247839/1‑139 (MQ=255)
                                                      gCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 <  2:1253314/98‑1 (MQ=255)
                                                      gCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 >  1:1253314/1‑98 (MQ=255)
                                                       cGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 >  1:578726/1‑97 (MQ=255)
                                                       cGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 <  2:578726/97‑1 (MQ=255)
                                                       cGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGt                                                                       >  2:1484850/1‑139 (MQ=255)
                                                         aaaCTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGc                                                                     >  2:446874/1‑139 (MQ=255)
                                                               tctTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGAc                                                                            >  1:550311/1‑126 (MQ=255)
                                                               tctTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGAc                                                                            <  2:550311/126‑1 (MQ=255)
                                                                         ttGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCggg                                                                                                  >  1:447920/1‑94 (MQ=255)
                                                                         ttGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCggg                                                                                                  <  2:447920/94‑1 (MQ=255)
                                                                                tatCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCCACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              <  2:1086117/139‑1 (MQ=255)
                                                                                tatCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              <  2:1184425/139‑1 (MQ=255)
                                                                                  tCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCa                                            >  2:1204949/1‑139 (MQ=255)
                                                                                      tctcAACCTGGAAGGTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAACGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGACTGACCCGCAGTTg                                        >  1:941517/1‑139 (MQ=255)
                                                                                      tctcAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTg                                        >  2:214868/1‑139 (MQ=255)
                                                                                          aaCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa                                    >  2:491430/1‑139 (MQ=255)
                                                                                           aCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACaa                                   <  2:1247839/139‑1 (MQ=255)
                                                                                               ggAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAAcc                               >  2:589225/1‑139 (MQ=255)
                                                                                                gAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCaa                                                               >  1:261586/1‑106 (MQ=255)
                                                                                                gAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCaa                                                               <  2:261586/106‑1 (MQ=255)
                                                                                                 aaGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCtt                             >  1:308287/1‑139 (MQ=255)
                                                                                                 aaGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCtt                             >  1:813879/1‑139 (MQ=255)
                                                                                                    aTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACg                                                                           >  1:605300/1‑90 (MQ=255)
                                                                                                    aTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACg                                                                           <  2:605300/90‑1 (MQ=255)
                                                                                                      ttCGTTCAGCGTGTGAATGCCGATATCGCTAACaa                                                                                                                                <  1:520118/35‑1 (MQ=255)
                                                                                                      ttCGTTCAGCGTGTGAATGCCGATATCGCTAACaa                                                                                                                                >  2:520118/1‑35 (MQ=255)
                                                                                                       tCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGa                       <  2:308287/139‑1 (MQ=255)
                                                                                                       tCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGa                       <  2:813879/139‑1 (MQ=255)
                                                                                                          ttCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           <  1:1431025/36‑1 (MQ=255)
                                                                                                          ttCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           >  2:1431025/1‑36 (MQ=255)
                                                                                                          ttCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGAcc                                                >  2:626422/1‑111 (MQ=255)
                                                                                                          ttCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGAcc                                                <  1:626422/111‑1 (MQ=255)
                                                                                                              gCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCt                <  1:214868/139‑1 (MQ=255)
                                                                                                               cGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAAACTTCACTGATGCCgacg               >  2:386993/1‑136 (MQ=255)
                                                                                                                 tgtgAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATg                                                                                                      <  1:1525660/50‑1 (MQ=255)
                                                                                                                 tgtgAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATg                                                                                                      >  2:1525660/1‑50 (MQ=255)
                                                                                                                 tgtgAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGtt                                         <  2:206216/111‑1 (MQ=255)
                                                                                                                 tgtgAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGtt                                         >  1:206216/1‑111 (MQ=255)
                                                                                                                           gATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTg   <  1:446874/139‑1 (MQ=255)
                                                                                                                           gATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTg   <  2:79601/139‑1 (MQ=255)
                                                                                                                           gATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTg   <  1:491430/139‑1 (MQ=255)
                                                                                                                            atatCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGa  <  2:941517/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                    
GCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGA  >  NC_000913/2195329‑2195591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A