Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,199,396 |
C→T |
pseudogene (75/948 nt) |
yehH → |
DUF4132 family pseudogene, defective yehI paralog, molybdate metabolism regulator, first fragment, molybdate metabolism regulator, second fragment 2, yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,199,396 | 0 | C | T | 100.0%
| 78.1
/ NA
| 26 | pseudogene (75/948 nt) | yehH | DUF4132 family pseudogene, defective yehI paralog, molybdate metabolism regulator, first fragment, molybdate metabolism regulator, second fragment 2, yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/13); total (13/13) |
TTGCCGACATTGCCGAGAGCCGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGA > NC_000913/2199268‑2199523
|
ttGCCGACATTGCCGAGAGCCGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCATTTTTGATGaaa < 1:863959/139‑1 (MQ=255)
cGAGAGCCGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGTATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGtt < 1:871040/139‑1 (MQ=255)
ccGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCTCGGCAGTTCACCGTCAGTTTtgatga > 2:727273/1‑118 (MQ=255)
ccGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTtgatga < 1:727273/118‑1 (MQ=255)
cgcgAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGTCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGt > 1:855356/1‑139 (MQ=255)
gcgAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGTCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTg < 2:855356/139‑1 (MQ=255)
aGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCtt > 2:561142/1‑104 (MQ=255)
aGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCtt < 1:561142/104‑1 (MQ=255)
ttGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTATGCGTGATGTTTCCGGCAGCCGCCTGa < 2:911909/139‑1 (MQ=255)
tGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGa < 1:1156952/138‑1 (MQ=255)
tGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGa > 2:1156952/1‑138 (MQ=255)
gggTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTtgtg < 2:1123952/105‑1 (MQ=255)
gggTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTtgtg > 1:1123952/1‑105 (MQ=255)
tCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAg < 2:1211846/102‑1 (MQ=255)
tCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAg > 1:1211846/1‑102 (MQ=255)
ggATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCgatgat > 2:483314/1‑139 (MQ=255)
tAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAAcg > 2:1185889/1‑139 (MQ=255)
aaCGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAAcgc > 2:3974/1‑139 (MQ=255)
ttCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTtgatga > 2:218430/1‑51 (MQ=255)
ttCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGTTTtgatga < 1:218430/51‑1 (MQ=255)
ctgctgctgGACTTTCGCGCTTGGCAGTTCACCGTCAGTTTTGATTAAGCCTTAGATCCGTTTCTGCGTGATGTTTCCGGCAGCCGCCTGGAAGACCGGGCCTCGCCGTACAAAAGCGATTATGAAACGCGGGCGAACg < 1:699744/139‑1 (MQ=255)
ctgGATTTTGGCCCACGGCAGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCgg > 2:530524/1‑139 (MQ=255)
aGTTCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCt < 1:483314/139‑1 (MQ=255)
ttCACCGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGa > 2:343627/1‑139 (MQ=255)
cGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAAc < 1:932162/118‑1 (MQ=255)
cGTCAGTTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAAc > 2:932162/1‑118 (MQ=255)
|
TTGCCGACATTGCCGAGAGCCGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGA > NC_000913/2199268‑2199523
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
