Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,438,548	T→C	V200A (GTG→GCG)	flk →	putative flagella assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,438,548	0	T	C	100.0%	178.8 / NA	50	V200A (GTG→GCG)	flk	putative flagella assembly protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/27); total (23/27)

CAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTA  >  NC_000913/2438412‑2438678

cagcagCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGa                                                                                                                                  >  1:250594/1‑139 (MQ=255)

      cgcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGATGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCGGGAACTTTCCGGGGCGAAAGATg                                                                                                                            >  2:866447/1‑139 (MQ=255)

       gcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAATCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATgg                                                                                                                           >  1:870000/1‑139 (MQ=255)

        cgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGc                                                                                                                          <  1:213794/139‑1 (MQ=255)

                ccgaccgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAAt                                                                                                                  <  1:530292/139‑1 (MQ=255)

                     cgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAg                                                                                                             >  2:957263/1‑139 (MQ=255)

                      gTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGc                                                                                                            >  2:990636/1‑139 (MQ=255)

                        ccTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGa                                                                                                          >  1:710497/1‑139 (MQ=255)

                            tattaCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACt                                                                                                      <  1:957263/139‑1 (MQ=255)

                               taCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGtt                                                                                                   <  2:580129/139‑1 (MQ=255)

                                  cggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGTAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTaa                                                                                                >  2:907317/1‑139 (MQ=255)

                                  cggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTaa                                                                                                >  1:46071/1‑139 (MQ=255)

                                   ggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAc                                                                                               >  2:1283150/1‑139 (MQ=255)

                                    gcggAGCACAATGCGCTAAAACAGCTGGTGACCAAATTTGCGGCGGCGACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAcc                                                                                              >  1:836359/1‑139 (MQ=255)

                                        aGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCt                                                                                          >  2:1051701/1‑139 (MQ=255)

                                          cacaATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTgg                                                                                        >  1:497520/1‑139 (MQ=255)

                                              aTGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    <  2:1256133/139‑1 (MQ=255)

                                              aTGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    <  2:1312268/139‑1 (MQ=255)

                                              aTGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    <  1:907317/139‑1 (MQ=255)

                                               tGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                                                                       >  1:1191758/1‑135 (MQ=255)

                                               tGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                                                                       <  2:1191758/135‑1 (MQ=255)

                                                gcgcTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCt                                                                                  <  2:870000/139‑1 (MQ=255)

                                                           gCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGgcg                                                                       >  1:1071992/1‑139 (MQ=255)

                                                                tGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCa                                                                  <  2:46071/139‑1 (MQ=255)

                                                                  aCCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAg                                                                                                             <  1:786815/94‑1 (MQ=255)

                                                                  aCCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAg                                                                                                             >  2:786815/1‑94 (MQ=255)

                                                                          tGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGc                                                        >  2:947540/1‑139 (MQ=255)

                                                                          tGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGc                                                        <  2:1071992/139‑1 (MQ=255)

                                                                            cggcggCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATgg                                                                                                                           >  1:134423/1‑70 (MQ=255)

                                                                            cggcggCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATgg                                                                                                                           <  2:134423/70‑1 (MQ=255)

                                                                                            aCCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACg                                      <  1:947540/139‑1 (MQ=255)

                                                                                              ccAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    <  1:435737/139‑1 (MQ=255)

                                                                                                 gCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCTGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGa                                 <  1:866447/139‑1 (MQ=255)

                                                                                                 gCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGa                                 <  2:1193653/139‑1 (MQ=255)

                                                                                                 gCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGa                                 <  2:250594/139‑1 (MQ=255)

                                                                                                  cAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTa                                                                                                 <  1:756477/74‑1 (MQ=255)

                                                                                                  cAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTa                                                                                                 >  2:756477/1‑74 (MQ=255)

                                                                                                   aaaCAGATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAAt                               <  1:1178148/139‑1 (MQ=255)

                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    >  1:919713/1‑81 (MQ=255)

                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                                                                    <  2:919713/81‑1 (MQ=255)

                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    <  2:251879/129‑1 (MQ=255)

                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    >  1:251879/1‑129 (MQ=255)

                                                                                                        gATCTGGCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTa                          <  1:990636/139‑1 (MQ=255)

                                                                                                              gCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    >  2:61636/1‑123 (MQ=255)

                                                                                                              gCAATCGATGCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCt                                    <  1:61636/123‑1 (MQ=255)

                                                                                                                       gCTGGAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTaaaa           <  1:445815/139‑1 (MQ=255)

                                                                                                                           gAACTTTCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAaacaac       <  2:710497/139‑1 (MQ=255)

                                                                                                                                ttCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                                                                       <  1:917785/54‑1 (MQ=255)

                                                                                                                                ttCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                                                                       >  2:917785/1‑54 (MQ=255)

                                                                                                                                ttCCGGGGCGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTa  >  1:983516/1‑139 (MQ=255)

CAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAATCACTACAGATGACGCTAAAACAACCTTTA  >  NC_000913/2438412‑2438678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A