Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,511,468	C→T	413 (TAG→TAA)	mntH ←	manganese/divalent cation transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,468	0	C	T	100.0%	140.3 / NA	44	413 (TAG→TAA)	mntH	manganese/divalent cation transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/22); total (22/22)

TCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTG  >  NC_000913/2511333‑2511594

tCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                             >  1:553758/1‑139 (MQ=255)

 ccGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACa                                                                                                                            >  2:1292382/1‑139 (MQ=255)

 ccGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACa                                                                                                                            >  2:187952/1‑139 (MQ=255)

           aGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAgcgc                                                                                                                  >  2:526640/1‑139 (MQ=255)

            gCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGcc                                                                                                                 <  1:746389/139‑1 (MQ=255)

             cacaATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCg                                                                                                                >  1:868576/1‑139 (MQ=255)

                 aTGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTccc                                                                                                            <  2:1015685/139‑1 (MQ=255)

                     cTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCccacc                                                                                                        >  2:498473/1‑139 (MQ=255)

                      tGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCccacca                                                                                                       <  1:1292382/139‑1 (MQ=255)

                            gACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAAcc                                                                                                 <  2:553758/139‑1 (MQ=255)

                              cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaa                                                                                                      >  2:628157/1‑132 (MQ=255)

                              cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaa                                                                                                      <  1:628157/132‑1 (MQ=255)

                              cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAg                                                                                               >  1:948824/1‑139 (MQ=255)

                              cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAg                                                                                               <  1:498473/139‑1 (MQ=255)

                                               aGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAc                                                                              <  1:526640/139‑1 (MQ=255)

                                                   cTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                                          >  1:1061354/1‑139 (MQ=255)

                                                     aCAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAgcg                                                                                                                   <  1:690144/96‑1 (MQ=255)

                                                     aCAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAgcg                                                                                                                   >  2:690144/1‑96 (MQ=255)

                                                           cGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                                                <  2:501099/125‑1 (MQ=255)

                                                           cGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                                                >  1:501099/1‑125 (MQ=255)

                                                                  ttAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAg                                                           <  1:458624/139‑1 (MQ=255)

                                                                        cgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtc                                                     <  1:375223/139‑1 (MQ=255)

                                                                        cgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtc                                                     <  2:341815/139‑1 (MQ=255)

                                                                           aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt                                                  >  1:266435/1‑139 (MQ=255)

                                                                                     aGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGtt                                        <  2:948824/139‑1 (MQ=255)

                                                                                       gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                                          >  2:992140/1‑103 (MQ=255)

                                                                                       gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                                          <  1:992140/103‑1 (MQ=255)

                                                                                            tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGtt                                 >  1:486522/1‑139 (MQ=255)

                                                                                                 cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaac                                                                                                     <  2:627131/66‑1 (MQ=255)

                                                                                                 cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaac                                                                                                     >  1:627131/1‑66 (MQ=255)

                                                                                                 cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                        <  1:1039897/95‑1 (MQ=255)

                                                                                                 cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                        >  2:1039897/1‑95 (MQ=255)

                                                                                                        gCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGcc                     <  2:114601/139‑1 (MQ=255)

                                                                                                          cAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCa                   <  1:187952/139‑1 (MQ=255)

                                                                                                                tGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                        <  1:513869/80‑1 (MQ=255)

                                                                                                                tGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                        >  2:513869/1‑80 (MQ=255)

                                                                                                                tGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATACTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGCGCGCCCATCAACt             >  2:1308047/1‑139 (MQ=255)

                                                                                                                tGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACAAGATATTCAGCGCCACGACCAGCACCACAATCCCCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACt             >  2:675988/1‑139 (MQ=255)

                                                                                                                        gCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCa     <  2:868576/139‑1 (MQ=255)

                                                                                                                          gACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTg                                      <  2:1297583/104‑1 (MQ=255)

                                                                                                                          gACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTg                                      >  1:1297583/1‑104 (MQ=255)

                                                                                                                           aCGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAc                                                                              <  1:1151263/63‑1 (MQ=255)

                                                                                                                           aCGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAc                                                                              >  2:1151263/1‑63 (MQ=255)

                                                                                                                           aCGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCGCCAGATCGCCCATCAACTTGCTGTCACTg  >  1:400498/1‑139 (MQ=255)

TCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTG  >  NC_000913/2511333‑2511594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A