Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,514,077	T→C	F346L (TTC→CTC)	nupC →	nucleoside (except guanosine) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,514,077	0	T	C	100.0%	182.8 / NA	52	F346L (TTC→CTC)	nupC	nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/27); total (25/27)

TTCCTTCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTG  >  NC_000913/2513943‑2514211

ttccttccAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCt                                                                                                                                    >  1:687341/1‑139 (MQ=255)

ttccttccAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTAGAGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGCAGGCATCATCTCTGTGCTCCt                                                                                                                                    >  2:626444/1‑139 (MQ=255)

          tGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTc                                                                                                                          >  1:209963/1‑139 (MQ=255)

           gAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCg                                                                                                                         <  1:243613/139‑1 (MQ=255)

                     aaGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTtctc                                                                                                               <  2:398888/139‑1 (MQ=255)

                         gggCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCa                                                                                                           <  2:209963/139‑1 (MQ=255)

                           gCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                         >  2:784428/1‑139 (MQ=255)

                           gCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                         <  1:626444/139‑1 (MQ=255)

                                 tCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGAt                                                                                                   >  1:347457/1‑139 (MQ=255)

                                    tGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTAt                                                                                                >  2:650829/1‑139 (MQ=255)

                                       cGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTctct                                                                                                              >  1:458430/1‑122 (MQ=255)

                                       cGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTctct                                                                                                              <  2:458430/122‑1 (MQ=255)

                                         aCCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAg                                                                                           >  2:738412/1‑139 (MQ=255)

                                                  gTTTGCAAGGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTa                                                                                  <  2:579169/139‑1 (MQ=255)

                                                            aGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtgaa                                                                        >  2:590470/1‑139 (MQ=255)

                                                              ttCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTaaa                                                                                <  2:183583/129‑1 (MQ=255)

                                                              ttCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTaaa                                                                                >  1:183583/1‑129 (MQ=255)

                                                                   tGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAagag                                                                 <  1:590470/139‑1 (MQ=255)

                                                                        tgatgGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAgg                                                            <  1:513106/139‑1 (MQ=255)

                                                                             gATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACg                                                       <  1:650829/139‑1 (MQ=255)

                                                                                   cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCtt                                                                                                             <  1:690108/79‑1 (MQ=255)

                                                                                   cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCtt                                                                                                             >  2:690108/1‑79 (MQ=255)

                                                                                   cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGttt                                                 >  1:805850/1‑139 (MQ=255)

                                                                                   cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGttt                                                 >  1:947845/1‑139 (MQ=255)

                                                                                    aGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTtc                                                <  2:947845/139‑1 (MQ=255)

                                                                                        aaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCt                                                                                                                            >  1:571267/1‑59 (MQ=255)

                                                                                        aaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCt                                                                                                                            <  2:571267/59‑1 (MQ=255)

                                                                                        aaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCa                                                               >  1:415641/1‑120 (MQ=255)

                                                                                        aaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCa                                                               <  2:415641/120‑1 (MQ=255)

                                                                                          ttGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCtt                                          <  1:738412/139‑1 (MQ=255)

                                                                                                          tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGttt                                                                                                                               >  2:823722/1‑38 (MQ=255)

                                                                                                          tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGttt                                                                                                                               <  1:823722/38‑1 (MQ=255)

                                                                                                          tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGt                                                                                         <  1:90019/76‑1 (MQ=255)

                                                                                                          tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGt                                                                                         >  2:90019/1‑76 (MQ=255)

                                                                                                          tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGtt                          >  2:859126/1‑139 (MQ=255)

                                                                                                            cgcgTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTa                        <  2:347457/139‑1 (MQ=255)

                                                                                                            cgcgTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTa                        <  2:805850/139‑1 (MQ=255)

                                                                                                                 gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  1:859126/139‑1 (MQ=255)

                                                                                                                 gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  2:577503/139‑1 (MQ=255)

                                                                                                                 gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  2:687341/139‑1 (MQ=255)

                                                                                                                 gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  1:359388/139‑1 (MQ=255)

                                                                                                                  cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                         >  2:212446/1‑52 (MQ=255)

                                                                                                                  cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                         <  1:212446/52‑1 (MQ=255)

                                                                                                                  cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGctc                  >  2:1054240/1‑139 (MQ=255)

                                                                                                                    gAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   >  1:805110/1‑136 (MQ=255)

                                                                                                                    gAAGGCATCATCTCTCTGCTCCTGCTTTCCTTCGCTAACTTCTCTTCAATCGTGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  2:805110/136‑1 (MQ=255)

                                                                                                                          atcatcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTgg          >  2:41456/1‑139 (MQ=255)

                                                                                                                            catcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtgaatgaa                                                                    >  1:262203/1‑79 (MQ=255)

                                                                                                                            catcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtgaatgaa                                                                    <  2:262203/79‑1 (MQ=255)

                                                                                                                               ctctGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAgt     >  2:334369/1‑139 (MQ=255)

                                                                                                                                  tgtgCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGtgtg  >  2:766224/1‑139 (MQ=255)

                                                                                                                                  tgtgCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGtgtg  <  1:20903/139‑1 (MQ=255)

TTCCTTCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTG  >  NC_000913/2513943‑2514211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A