Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,518,271 |
G→A |
intergenic (‑40/‑196) |
alaW ← / → yfeC |
tRNA‑Ala/DUF1323 family putative DNA‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,518,271 | 0 | G | A | 100.0%
| 134.5
/ NA
| 42 | intergenic (‑40/‑196) | alaW/yfeC | tRNA‑Ala/DUF1323 family putative DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/20); total (22/20) |
CTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAGTGGTG > NC_000913/2518142‑2518404
|
cTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGcc > 1:1211721/1‑139 (MQ=255)
gTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGaa > 1:309743/1‑139 (MQ=255)
tGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGc < 1:1187354/133‑1 (MQ=255)
tGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGc > 2:1187354/1‑133 (MQ=255)
ggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCaa > 2:384687/1‑139 (MQ=255)
cTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTg > 1:665331/1‑139 (MQ=255)
gggATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgt < 1:384687/139‑1 (MQ=255)
aaCCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGttt > 2:912108/1‑139 (MQ=255)
cTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgtg < 2:1267035/128‑1 (MQ=255)
cTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgtg > 1:1267035/1‑128 (MQ=255)
tGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCg < 2:1211721/139‑1 (MQ=255)
gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAgg < 1:432080/139‑1 (MQ=255)
tgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCaaa > 1:432657/1‑139 (MQ=255)
aGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGaa < 2:432657/139‑1 (MQ=255)
gCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGaaa > 2:266702/1‑139 (MQ=255)
tataGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTc > 2:318467/1‑139 (MQ=255)
cccGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTc < 2:665331/139‑1 (MQ=255)
cGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATgtgt > 2:827531/1‑59 (MQ=255)
cGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATgtgt < 1:827531/59‑1 (MQ=255)
cGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCag < 1:1094836/139‑1 (MQ=255)
gTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCagt > 2:1030000/1‑139 (MQ=255)
gTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCagt > 1:175356/1‑139 (MQ=255)
gTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCagt < 2:309743/139‑1 (MQ=255)
gTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTa > 2:580691/1‑139 (MQ=255)
tAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgtgt > 2:542855/1‑73 (MQ=255)
tAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgtgt < 1:542855/73‑1 (MQ=255)
gTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCa < 1:1327878/59‑1 (MQ=255)
gTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCa > 2:1327878/1‑59 (MQ=255)
tCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCAc > 2:967968/1‑139 (MQ=255)
tCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCAc > 1:153913/1‑139 (MQ=255)
aGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGt < 1:434220/139‑1 (MQ=255)
tGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTc < 2:175356/139‑1 (MQ=255)
gACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTc > 1:786602/1‑125 (MQ=255)
gACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTc < 2:786602/125‑1 (MQ=255)
ggcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAg < 1:580691/139‑1 (MQ=255)
ggcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAg < 1:266702/139‑1 (MQ=255)
ggcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAg > 1:257568/1‑139 (MQ=255)
gcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGc < 2:1152086/74‑1 (MQ=255)
gcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGc > 1:1152086/1‑74 (MQ=255)
catcatATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAgtg < 1:318467/139‑1 (MQ=255)
tcatATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAgtggt > 2:121459/1‑139 (MQ=255)
catATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAgtggtg < 2:153913/139‑1 (MQ=255)
|
CTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAGTGGTG > NC_000913/2518142‑2518404
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
