Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,958,309	T→C	A192A (GCA→GCG)	ptrA ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,958,309	0	T	C	100.0%	123.8 / NA	35	A192A (GCA→GCG)	ptrA	protease III
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/16); total (19/16)

TAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTC  >  NC_000913/2958178‑2958434

tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGt                                                                                                                        >  1:583418/1‑139 (MQ=255)

       ccGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGt                                                                                                                 >  1:531366/1‑139 (MQ=255)

          ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTgcgc                                                                                                                             >  1:1059344/1‑124 (MQ=255)

          ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTc                                                                                                              >  2:1133005/1‑139 (MQ=255)

          ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTc                                                                                                              >  1:422781/1‑139 (MQ=255)

          ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTc                                                                                                              >  1:586243/1‑139 (MQ=255)

          ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGAGTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTc                                                                                                              >  1:780384/1‑139 (MQ=255)

                     ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCt                                                                                                   >  2:443990/1‑139 (MQ=255)

                                      ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCg                                                                                  >  1:256664/1‑139 (MQ=255)

                                        cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCAt                                                                                      >  1:100373/1‑133 (MQ=255)

                                        cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCAt                                                                                      <  2:100373/133‑1 (MQ=255)

                                        cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                 >  2:329900/1‑138 (MQ=255)

                                        cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                 <  1:329900/138‑1 (MQ=255)

                                        cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTc                                                                                >  1:157510/1‑139 (MQ=255)

                                        cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTc                                                                                >  2:190571/1‑139 (MQ=255)

                                           ggAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCAcg                                                                             >  1:1032681/1‑139 (MQ=255)

                                               aTCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                         <  2:1016810/139‑1 (MQ=255)

                                                   ttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGa                                                                                                      <  2:518887/106‑1 (MQ=255)

                                                   ttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGa                                                                                                      >  1:518887/1‑106 (MQ=255)

                                                          ccTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGttt                                                                                                               >  1:401565/1‑90 (MQ=255)

                                                          ccTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGttt                                                                                                               <  2:401565/90‑1 (MQ=255)

                                                                   ccGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGc                                                     <  1:443990/139‑1 (MQ=255)

                                                                      gATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCAt                                                                                            <  1:2047/97‑1 (MQ=255)

                                                                      gATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCAt                                                                                            >  2:2047/1‑97 (MQ=255)

                                                                      gATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGtt                                                  <  1:326244/139‑1 (MQ=255)

                                                                       aTTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTc                                                 <  2:586243/139‑1 (MQ=255)

                                                                                   gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGc                                     >  2:499791/1‑139 (MQ=255)

                                                                                    tCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                    <  2:750009/139‑1 (MQ=255)

                                                                                           aaGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTc                             <  2:256664/139‑1 (MQ=255)

                                                                                              tttCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGc                          <  1:190571/139‑1 (MQ=255)

                                                                                                  gAGGTTACCACCAGAAAACTTTGAACCGGGGTgcgc                                                                                                                             <  2:659769/36‑1 (MQ=255)

                                                                                                         ccaccaGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCATCGCATTACGCTCACGTTCGGCATATTTCTTGt               <  2:780384/139‑1 (MQ=255)

                                                                                                                 aaaCTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCaaa       <  2:531366/139‑1 (MQ=255)

                                                                                                                    cTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGt    >  2:1098972/1‑139 (MQ=255)

                                                                                                                      ttGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTc  <  2:1032681/139‑1 (MQ=255)

                                                                                                                       tGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                   <  1:1178543/73‑1 (MQ=255)

                                                                                                                       tGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                   >  2:1178543/1‑73 (MQ=255)

TAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTC  >  NC_000913/2958178‑2958434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A