Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,021	T→C	Y35C (TAC→TGC)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,021	0	T	C	100.0%	231.6 / NA	63	Y35C (TAC→TGC)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (32/31); total (32/31)

ACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGAAA  >  NC_000913/2983888‑2984155

acCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCa                                                                                                                                   >  2:756607/1‑139 (MQ=255)

  cTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATc                                                                                                                                 >  2:1084027/1‑139 (MQ=255)

     aCTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGc                                                                                                                              >  2:1243509/1‑139 (MQ=255)

          cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACaa                                                                                                                         >  1:1128457/1‑139 (MQ=255)

          cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACaa                                                                                                                         >  2:1239013/1‑139 (MQ=255)

          cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACaa                                                                                                                         >  2:1260931/1‑139 (MQ=255)

                   aGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCtttt                                                                                                                <  1:30453/139‑1 (MQ=255)

                         tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCgg                                                                                                                               >  2:1268911/1‑118 (MQ=255)

                         tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCgg                                                                                                                               <  1:1268911/118‑1 (MQ=255)

                         tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCa                                                                                                          >  2:987410/1‑139 (MQ=255)

                         tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCa                                                                                                          >  2:1280133/1‑139 (MQ=255)

                            cTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGGGCACTCATCGGCGACAAATACCTTTTCAACCaaaa                                                                                                       >  1:795123/1‑139 (MQ=255)

                                cGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCTCTCATCGGCGACAAATACCTTTTCAACCAAAAATTc                                                                                                   >  1:323500/1‑139 (MQ=255)

                                    gAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTg                                                                                               >  2:32649/1‑139 (MQ=255)

                                      gttgttTACCAACTTCCCCGCCAACGGAAGCCGTTTTAGGTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  2:1050916/139‑1 (MQ=255)

                                      gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCTCTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  2:323500/139‑1 (MQ=255)

                                      gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:315402/139‑1 (MQ=255)

                                      gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:756607/139‑1 (MQ=255)

                                       ttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcgc                                                                                            <  1:32649/139‑1 (MQ=255)

                                         gttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAg                                                                                          <  2:925428/139‑1 (MQ=255)

                                           ttACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGcc                                                                                        <  2:1128457/139‑1 (MQ=255)

                                                  cTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTGAACCATGGTGCACTCATCGGCGACAATTACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGt                                                                                 >  1:797950/1‑139 (MQ=255)

                                                  cTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGAc                                                                                                                           <  2:1095255/97‑1 (MQ=255)

                                                  cTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGAc                                                                                                                           >  1:1095255/1‑97 (MQ=255)

                                                    tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACa                                                                                                                          >  2:9061/1‑96 (MQ=255)

                                                    tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACa                                                                                                                          <  1:9061/96‑1 (MQ=255)

                                                    tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             >  2:300581/1‑125 (MQ=255)

                                                    tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:300581/125‑1 (MQ=255)

                                                      cccGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATcc                                                                             >  1:267588/1‑139 (MQ=255)

                                                              cGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGtttt                                                                     <  1:461177/139‑1 (MQ=255)

                                                                   aCCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTtgcgt                                                                >  2:244/1‑139 (MQ=255)

                                                                      gTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTtgcgtgcg                                                             <  1:649836/139‑1 (MQ=255)

                                                                              tATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTgg                                                     <  1:457899/139‑1 (MQ=255)

                                                                               aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                  >  1:1252128/1‑93 (MQ=255)

                                                                               aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                  <  2:1252128/93‑1 (MQ=255)

                                                                               aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGa                                                    >  1:299065/1‑139 (MQ=255)

                                                                                tCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGAt                                                   <  1:549351/139‑1 (MQ=255)

                                                                                tCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGAt                                                   <  2:267588/139‑1 (MQ=255)

                                                                                 cGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATg                                                  <  1:1084027/139‑1 (MQ=255)

                                                                                  ggcttcaTGCCGTCGACAATCATCCGGTTAATGTGGCTGTAAACCACGGTGCACTCATCGGCGACAAATACCATTTCACCCACAAAATCGTTAGGCATGACCTGGGTATACAGCGTATTTGCGTGCGCCCTGCGGacgt                                                 <  2:188290/133‑4 (MQ=255)

                                                                                  ggCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGc                                                 <  1:1243509/139‑1 (MQ=255)

                                                                                         tGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                  >  1:1283403/1‑83 (MQ=255)

                                                                                         tGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                  <  2:1283403/83‑1 (MQ=255)

                                                                                         tGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCt                                          <  1:1239013/139‑1 (MQ=255)

                                                                                              ccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGt                                     >  2:234203/1‑139 (MQ=255)

                                                                                                        ttCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAAc                           <  2:299065/139‑1 (MQ=255)

                                                                                                            gTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTcc                       >  1:546801/1‑139 (MQ=255)

                                                                                                                     gCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAg              <  2:1113763/139‑1 (MQ=255)

                                                                                                                     gCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAg              >  1:938907/1‑139 (MQ=255)

                                                                                                                      cTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGt             >  2:834119/1‑139 (MQ=255)

                                                                                                                         tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             >  2:579141/1‑56 (MQ=255)

                                                                                                                         tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             >  2:1216958/1‑56 (MQ=255)

                                                                                                                         tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:579141/56‑1 (MQ=255)

                                                                                                                         tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                             <  1:1216958/56‑1 (MQ=255)

                                                                                                                         tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGcc                                                                                        >  1:310430/1‑61 (MQ=255)

                                                                                                                         tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGcc                                                                                        <  2:310430/61‑1 (MQ=255)

                                                                                                                          aaaCCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAg         <  1:234203/139‑1 (MQ=255)

                                                                                                                           aaCCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTa                                                                                <  2:246099/67‑1 (MQ=255)

                                                                                                                           aaCCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTa                                                                                >  1:246099/1‑67 (MQ=255)

                                                                                                                                tGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaa   >  2:735348/1‑139 (MQ=255)

                                                                                                                                tGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaa   <  1:244/139‑1 (MQ=255)

                                                                                                                                 ggTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaaa  >  1:1191146/1‑139 (MQ=255)

                                                                                                                                 ggTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaaa  >  1:1095404/1‑139 (MQ=255)

ACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGAAA  >  NC_000913/2983888‑2984155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A