Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
766 |
55.6 |
2631458 |
94.7% |
2491990 |
109.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,328,103 |
A→C |
intergenic (+20/+136) |
yhbY → / ← greA |
RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,328,103 | 0 | A | C | 100.0%
| 106.7
/ NA
| 30 | intergenic (+20/+136) | yhbY/greA | RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (13/17); total (13/17) |
CCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTA > NC_000913/3327968‑3328234
|
ccTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCAt < 1:1211907/139‑1 (MQ=255)
ccTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCAt < 1:887292/139‑1 (MQ=255)
cgcgAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGATAAGATTAACCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGttt > 2:359159/1‑139 (MQ=255)
aCCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCgct < 1:772453/139‑2 (MQ=255)
gcgcCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACCCCATTTGCTGTGTAAAACGAGGGGTTTTCCgcaggc < 2:1161294/139‑1 (MQ=255)
ggTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCa < 2:959007/139‑1 (MQ=255)
gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTg < 1:285358/92‑1 (MQ=255)
gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTg > 2:285358/1‑92 (MQ=255)
gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCAc > 2:724157/1‑139 (MQ=255)
gTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCAc > 2:1157721/1‑139 (MQ=255)
cGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGctct > 2:978145/1‑139 (MQ=255)
aaaaCGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTc < 2:998972/139‑1 (MQ=255)
aaCGCTGGTGCTTTATCGCGCAACTAAAGAACGTAAAATCGCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCAGTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGt < 2:1278200/139‑1 (MQ=255)
cTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGAt > 2:131520/1‑139 (MQ=255)
cccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAg > 2:61203/1‑139 (MQ=255)
cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAGAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCa < 2:201618/99‑1 (MQ=255)
cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAGAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCa > 1:201618/1‑99 (MQ=255)
aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttt < 1:1157721/139‑1 (MQ=255)
cGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct < 1:978145/139‑1 (MQ=255)
cGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct < 1:61203/139‑1 (MQ=255)
tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCcttt > 2:306289/1‑139 (MQ=255)
aaaaTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttc > 1:318393/1‑139 (MQ=255)
cGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACaa < 1:306289/139‑1 (MQ=255)
tGCCACGCTAAGATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATAc > 1:73966/1‑139 (MQ=255)
gATTATCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCtt < 2:318393/139‑1 (MQ=255)
tCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTa > 2:1165230/1‑139 (MQ=255)
tCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTa < 1:724157/139‑1 (MQ=255)
tCCTAAAGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTa < 1:1165230/139‑1 (MQ=255)
aGTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGt > 1:1215276/1‑139 (MQ=255)
gTTACA‑CCCATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTa < 2:1215276/139‑1 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGctc < 2:571478/62‑1 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGctc > 1:571478/1‑62 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttttt > 2:428649/1‑97 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttttt < 1:428649/97‑1 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct < 1:662744/100‑1 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct > 2:47685/1‑100 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct > 2:662744/1‑100 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct < 1:47685/100‑1 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACaa < 1:971980/110‑1 (MQ=255)
aca‑cccATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACaa > 2:971980/1‑110 (MQ=255)
ca‑cccaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGcc < 2:249876/91‑1 (MQ=255)
ca‑cccaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGt < 2:566865/134‑1 (MQ=255)
|
CCTTGATCGTGGAAGCTATCGTGCGCGAAACCGGCGCCTGTAATGTACAGGTCATCGGTAAAACGCTGGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACA‑CACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTA > NC_000913/3327968‑3328234
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
